下颌牵张成骨中应用神经生长因子对神经损伤修复的作用.docVIP

下颌牵张成骨中应用神经生长因子对神经损伤修复的作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：下颌牵张成骨中应用神经生长因子对神经损伤修复的作用 1 1 实验动物及分组 3 2 牵张器 4 3 手术过程 4 4 术后处理 4 5 神经电生理检查 5 文2：神经生长因子对猴基底前脑神经元的保护作用 9 一、实验动物 10 二、手术方法 医学 论文 网 10 三、组织学检查 10 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 下颌牵张成骨中应用神经生长因子对神经损伤修复的作用 文1：下颌牵张成骨中应用神经生长因子对神经损伤修复的作用 Recovery effect of systemic injection of nerve growth factor on inferior alveolar nervein a rabbit model of mandibular distraction osteogenesis Abstract： Objective To study the recovery effect of the inferior alveolar nerve following systemic injection of NGF (nerve growth factor) in a rabbit model of mandibular distraction Totally 48 New Zealand white rabbits underwent bilateral distraction osteogenesis with a rate of /12h for 10 after surgery,μg/d×20d NGF or normal saline were intramuscularly injected 420F monitoring system was applied to record the seory nerve action potentials(SNAP) before surgery,at the end of distraction,or at coolidation time of 1,2,4 weeks At the end of distraction and 1 week following distraction,the amplitude of the evoked potentials and latency showed no statistical difference between the NGF group and control group;at the coolidation time of 2,4 weeks,the amplitude of the evoked potentials and latency was significantly higher and lower respectively in NGF group than that in control group(P).Conclusion Systematic injection of NGF can help to accelerate the function recovery of inferior alveolar nerve in mandibular distraction osteogenesis. Key words：nerve growth factor;distraction osteogenesis;seory nerve action potential;inferior alveolar nerve 牵张成骨技术(distraction osteogenesis,DO)又称为“自体性骨组织工程”，是20世纪50 年代由前苏联学者Ilizarov[1] 发展 和完善并用于矫治各类四肢长骨畸形的一种方法，90年代后开始应用于口腔颌面外科域[2]，且发展迅速，已成为这一领域的研究热点之一。下颌骨有特殊的解剖结构：下齿槽神经(inferior alveolar nerve，IAN)位于骨性下颌管中，在牵张器植入、下颌骨牵引延长的过程中受到创伤和牵拉，这在一定程度上造成了IAN的损伤[3-5]。神经生长因子(nerve growth factor,NGF)是1952 年最先在研究鸡胚的神经发育过程中发现的。NGF对神经元的存活、生长发育、分化、再生和功能维持起调控作用。现已证实NGF是参与损伤神经再生和功能修复的重