不同品系来源的骨髓间充质干细胞对小鼠肾缺血再灌注损伤作用的对比性研究临床医学.docVIP

不同品系来源的骨髓间充质干细胞对小鼠肾缺血再灌注损伤作用的对比性研究临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：不同品系来源的骨髓间充质干细胞对小鼠肾缺血再灌注损伤作用的对比性研究临床医学 1 1 材料与方法 3 2 结 果 4 3 讨 论 5 文2：西汀对小鼠肾缺血再灌注损伤的保护 7 1　材料与方法 7 2　结　　果 9 3　讨论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 不同品系来源的骨髓间充质干细胞对小鼠肾缺血再灌注损伤作用的对比性研究临床医学 文1：不同品系来源的骨髓间充质干细胞对小鼠肾缺血再灌注损伤作用的对比性研究临床医学 【Abstract】 Objective To explore whether bone marrow mesenchymal stem cells (BMSCs) from different strain mice can promote repairing renal ischemiareperfusion (I/R) injury and provide a method for the treatment of renal diseases. Methods BMSCs were cultured and expanded from healthy 3weekold C57BL/6J mice and ICR mice in vitro, respectively. 8weekold C57BL/6J mice and ICR mice were randomly divided into sham, parallel control and cell traplantation groups, and 12 mice in each group. The renal I/R injury model was established. BMSCs were traplanted into cell traplantation group mice via the tail vein and saline was injected into parallel control group. Then, renal function, morphologic changes and renal pathology changes were detected after 2 weeks. The recovery of kidney of different strai mice were observed. Results BMSCs not only had a high purification, homogeneity and stability, but also had unlimited proliferation. The 57BL/6J mice in traplantation group appeared the good state, and the indexes of renal function were significantly improved (P＜), the renal histomorphology became better. The ICR mice in traplantation group appeared the poor state, the indexes of renal function were not different from those in control group, and some indexes were significantly higher than those of control group (P＞), the renal pathological changes were not improved. Conclusio The recovery of renal I/R injury in the host is improved after traplantation of BMSCs into C57BL/6J mice by tail vain and renal injury is improved. But BMSCs can not improve ICR mice renal I/R injury, or even exacerbate the extent of renal injury. 【Key words】 Bon