诱导骨髓间充质干细胞再生汗腺组织的神经与血管支配研究.docVIP

诱导骨髓间充质干细胞再生汗腺组织的神经与血管支配研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：诱导骨髓间充质干细胞再生汗腺组织的神经与血管支配研究 1 1 一般资料 3 2 诱导MSCs表型改变与移植 3 3 发汗试验及标本留取 4 4 免疫组织化学染色 4 5 统计学分析 5 1 诱导MSCs转分化为汗腺样细胞 5 2 发汗试验 5 3 免疫组织化学染色 5 文2：运用核磁共振SPGR序列研究三叉神经与血管 9 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 诱导骨髓间充质干细胞再生汗腺组织的神经与血管支配研究 文1：诱导骨髓间充质干细胞再生汗腺组织的神经与血管支配研究 Distribution of nerve and blood vessels in regenerative sweat glands Abstract： Objective To explore the distribution characteristics of nerve and blood vessels in regenerative sweat glands induced from bone marrow mesenchymal stem cells (MSCs) and confirm whether these sweat gland tissues are dominated by the newborn nerve and blood Four postburn patients who were operated with scar excision were enrolled in this sca were excised and two parts of these excised wounds were identified in every of them was used as the traplant and the other one taken as was taken the induced MSCs which were cocultured with sweat gland cells were implanted into scarcut they were covered with a piece of cellular allogeneic wounds in the control part were treated with the same procedures,but without cell test with iodine and starch was made after 3 months and the biopsy was taken from the traplanted areas and control immunohistochemistry was used to test the expression of S100(S100),neuron specific enolase(E),factor Ⅷ related antigen (ⅧR Ag) and expression of these facto in normal skin and scar tissue was also examined and used as The pepiration test on the traplanted area was positive,but negative in the control parts in four examination confirmed that the positive expression of S100,E,ⅧR Ag and CD34 could be found in tissues taken from the traplanted area,control parts,normal skin and scar ,the distributio of S100,E,ⅧR Ag and CD34 on the traplanted areas were similar to those on the normal The regenerative sweat glands from the induced MSCc have the distribution of the nerve and blood vessels and these funct