Survivin和p27Kip1蛋白在结肠癌中的表达及临床意义.docVIP

Survivin和p27Kip1蛋白在结肠癌中的表达及临床意义 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：Survivin和p27Kip1蛋白在结肠癌中的表达及临床意义 1 1 材料与方法 2 2 结 果 3 3 讨 论 4 文2：Survivin蛋白在肺癌中的表达及临床意义 6 1 材料与方法 7 参考文摘引言： 7 原创性声明（模板） 9 文章致谢（模板） 10 正文 Survivin和p27Kip1蛋白在结肠癌中的表达及临床意义 文1：Survivin和p27Kip1蛋白在结肠癌中的表达及临床意义 [Abstract] Objective To investigate the expression of Survivin and its correlation with expression of p27Kip1 protein in colon carcinoma. Methods Expression of Survivin and p27Kip1 protein was investigated by immunohistochemistry in 10 cases of normal colon tissues and 83 cases of colon cancer tissues. The relatiohip between Survivin expression and clinicopathological paramete as well as p27Kip1 expression was analyzed. Results The positive expression rate of Survivin was significantly higher in colon cancer tissue (%, 69/83) than in normal colon tissue (%, 0/10) (P). Expressio of Survivin were not related to the clinicopathological paramete (P). The positive expression rate of p27Kip1 was significantly lower in colon cancer tissue (%, 34/83) than in normal colon tissue (%, 9/10) (P). The positive expression rate of p27Kip1 in colon cancer was significantly lower in distant metastasis group (%, 3/18) than in nondistant metastasis group (%, 31/65) (P). The expression of p27Kip1 was significantly correlated with histological differentiation, invasion depth and Dukes stages (P). There was not correlation between the expression of Survivin and p27Kip1 (r=，P).Conclusion The abnormal expression of Survivin and p27Kip1 protein indicates that Survivin and p27Kip1 may play an important role in oet and progression of colon cancer. No correlation is found between Survivin and p27Kip1 expression in colon cancer. [Key words] Colon carcinoma; Immunohistochemistry; Survivin; p27Kip1 结直肠癌是最常见的消化道癌瘤之一,严重威胁人类健康。Survivin是近来发现的抑制凋亡基因家族（IAPs）的新成员。p27Kip1是一个周期素依赖性激酶抑制蛋白，是细胞周期G1期向S期转换的负向调控因子。 文献 未见关于结肠癌中Survivin与p27Kip1相关性的研究报道，两者在结肠癌中的关系尚未明确。本研究采用免疫组化法检测了结肠癌中Survivin和p27Kip1的表达，探讨两者在结肠癌中的表达