广藿香愈伤组织诱导和分化再生植株的研究.docVIP

广藿香愈伤组织诱导和分化再生植株的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 2 文1：广藿香愈伤组织诱导和分化再生植株的研究 2 1材料和方法 3 11材料试验 3 12方法 4 121外植体培养 4 122愈伤组织的诱导 4 123愈伤组织的分化 4 124无根苗的诱导 4 13培养条件 5 2结果与分析 5 21愈伤组织的诱导 5 211不同植物激素对愈伤组织诱导的影响 5 212不同浓度BA对愈伤组织诱导的影响 6 213不同外植体诱导愈伤组织能力的差异 6 22愈伤组织的增殖 7 221不同植物激素对愈伤组织增殖的影响 7 222愈伤组织培养时间对增殖的影响 7 23愈伤组织的分化 8 231不同植物激素对愈伤组织分化的影响 8 232不同外植体诱导的愈伤组织分化能力的差异 8 24无根苗诱导生根及移栽 9 文2：瓦松愈伤组织诱导研究 10 1 材料与仪器 10 2 方法 11 3 结果 12 4 讨论 12 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 广藿香愈伤组织诱导和分化再生植株的研究 文1：广藿香愈伤组织诱导和分化再生植株的研究 Abstract: ObjectiveCallus induction and plantlets regeneration of Pogostemon cablin(Blanco)Benth. are studied to establish a highefficiency regeneration system for tissue leaf segments, nodular stem segments, stem segments and root tips from Pogostemon cablin(Blanco)Benth. were cultured in vitro. The facto which affect the induction，multiplication and differentiation of callus were studied. ResultsBenzylaminopurine (BA) or thidiazuron (TDZ) combined with αnaphthylacetic acid (NAA) was helpful to induce callus. The optimal concentratio of BA were 02～05mg/L. Nodular stem segments and stem segments were the most suitable explants for callus induction. The medium containing BA and NAA promoted the multiplication of callus. The multiplication rate and differentiation rate of 30day callus were much higher than those of 60day callus. BA was helpful to induce callus differentiation. The callus from leaf segments exerted a higher differentiation rate. ConclusionA highefficiency regeneration system of callus induction and plant regeneration from Pogostemon cablin(Blanco) been established. Key words:POGOSTEMON CABLIN(BLANCO) BENTH./growth development;INVITRO CULTURE;CALLUS 广藿香［Pogostemon cablin (Blanco) Benth.］为唇形科刺蕊草属植物，以干燥地上部分入药，其性辛、微温。具有芳香化湿，开胃止呕，发表解暑的功效［1］。广藿香为广东道地药材，“十大广药”之一，是多种著名中成药“藿香正气丸”、“藿胆丸”等以及 现代 中药制剂“抗病毒口服液”的主要原料。广藿香原产于菲律宾、马来西亚等国，最早自宋代引