微生物学英文教学课件：chapter1 The Main Themes of Microbiology.ppt

It is not an overstatement to say that he and his colleagues invented many useful techniques : inoculation,isolation, media, maintenance of pure cultures, and preparation of specimens for microscopic examination. During Koch’s studies on bacterial diseases, it became necessary to isolate suspected bacterial pathogens. At first he cultured bacteria on the sterile surfaces of cut, boiled potatoes. This was unsatisfactory because bacteria would not always grow well on potatoes. He then tried to solidify regular liquid media by adding gelatin. Separate bacterial colonies developed afte