双黄连口服液联合庆大霉素的体外抗菌作用研究.docVIP

双黄连口服液联合庆大霉素的体外抗菌作用研究 摘要 目的：研究双黄连口服液和庆大霉素联用对金黄色葡萄球菌、铜绿假单胞菌的体外抗菌作用。方法：根据美国临床实验室标准化委员会(NCCLS)介绍的方法，使用微量溶液稀释法，测得双黄连口服液和庆大霉素分别对金黄色葡萄球菌、铜绿假单胞菌的最低抑菌浓度（minimum inhibitory concentration, MIC）;使用棋盘联合药敏试验法，以部分抑菌浓度（fractional inhibitory concentration，FIC）指数为指标，观察双黄连口服液和庆大霉素联用分别对金黄色葡萄球菌、铜绿假单胞菌的抗菌作用。结果：双黄连口服液对金黄色葡萄球菌和铜绿假单胞菌的MIC分别是46.88mg·ml-1和375 mg·ml-1;庆大霉素对金黄色葡萄球菌和铜绿假单胞菌的MIC分别是0.25U·ml-1和1 U·ml-1；双黄连口服液和庆大霉素联用对金黄色葡萄球菌和绿脓杆菌的FIC指数均是0.56。结论：双黄连口服液联用庆大霉素对金黄色葡萄球菌或铜绿假单胞菌均呈相加作用。 关键词：双黄连口服液；庆大霉素；抗菌作用；FIC指数 Study on Antibacterial Activity of Shuanghuanglian Syrup and Gentamicin Combination in vitro Abstract Objective：To explore the antibacterial effect of Shuanghuanglian Syrup combining with Gentamicin in vitro against Staphylococcus aureus and Pseudomonas aeruginosa . Methods：according to the standards of NCCLS, The MIC values of Shuanghuanglian Syrup and Gentamicin on Staphylococcus aureus and Pseudomonas aeruginosa were detected by microamount dilution method．The antibacterial activity of Shuanghuanglian Syrup and Gentamicin combination in vitro were determined by chessboard dilution method．The property of Shuanghuanglian Syrup and Gentamicin combination was assessed according to FIC index．Results：MIC of Shuanghuanglian Syrup on Staphylococcus aureus or Pseudomonas aeruginosa is respectively 46.88mg·mL-1and 375 mg·mL-1. MIC of Gentamicin on Staphylococcus aureus or Pseudomonas aeruginosa is respectively 0.25 U·mL-1 and 1 U·mL-1. FIC index of Shuanghuanglian Syrup combing with Gentamicin on Staphylococcus aureus or Pseudomonas aeruginosa is 0.56. Conclusion：Antibacterial effect of Shuanghuanglian Syrup combing with Gentamicin were additive effect on Staphylococcus aureus or Pseudomonas aeruginosa by antimicrobial susceptibilities test. Key words Shuanghuanglian Syrup, Gentamicin, antibacterial effect, FIC index. . 双黄连口服液具有辛凉解表、清热解毒功效，临床上主要用于上呼吸道感染、扁桃体炎、咽炎、病毒性肺炎等细菌和病毒感染性疾病[1]。其主要成分为金银花、黄芩、连翘，这些成分属清热解毒的中药，均有一定的抗菌作用[2--5]。庆大霉素因其高效低毒在临床上广泛应用，但是近年来随着临床应用的广泛，致病菌耐药性发生的严重性影响了其药效