人源化CD25单克隆抗体对外周血T淋巴细胞活化和.docVIP

人源化CD25单克隆抗体对外周血T淋巴细胞活化和 文档信息 ： 文档作为关于“医学心理学”中“肿瘤学”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文8426字，doc格式，可编辑。质优实惠，欢迎下载！ 正文 人源化CD25单克隆抗体对外周血T淋巴细胞活化和 文1：人源化CD25单克隆抗体对外周血T淋巴细胞活化和 effects of humanized recombinant cd25 monoclonal antibody on activation and proliferation of t lymphocytes liu wei， ren hanyun department of hematology，the fit hospital， peking univeity， beijing 100034， china abstract the study was purposed to investigate the effects of humanized recombined cd25 monoclonal antibody (rhcd25mab) on activation and proliferation of t lymphocytes in vitro. peripheral blood mononuclear cells (pbmnc) were incubated with phytohemagglutinin (pha). before or after t cell activation， the cells were cultured with or without rhcd25mab or cyclosporine a (csa) in vitro. after 72 hou incubation， the proliferation of lymphocytes was analyzed by mtt assay. the expression of cd3 and cd25 antige on t lymphocytes were detected by flow cytometry. the levels of sil2r in the supernatants were determined with elisa. the results showed that both rhcd25mab and csa could inhibit the proliferation of t lymphocytes significantly in concentrationdependent manner and csa was more efficient than rhcd25mab. both rhcd25mab and csa could also decrease the levels of sil2r in the supernatant and inhibit the expression of cd25 antigen on t lymphocytes. the level of sil2r and the expression of cd25 on t lymphocytes decreases more profoundly in rhcd25mab group. it is concluded that rhcd25mab shows strong immunosuppressive activity both before and after t cell activation， suggesting that this agent may be useful in not only prophylaxis but also the treatment of acute graftveushost disease。 key words humanized recombined cd25 monoclonal antibody; t lymphocyte; sil2r; cell surface antigen j exp hematol 2007; 15(1):134-137 rhcd25mab是一种新型的基因工程抗体，主要用于预防器官移植术后的急性排异反应［1］，近年在治疗异基因造血干细胞移植术后的移植物抗宿主病（gvhd）中也具有良好疗效［2-5］。（il2）受体α亚单位特异性结合，从而阻断il2与il2r的结合，抑制t细胞活化而发挥免疫抑制作用。本研究观察了该抗体在体外对t细胞增殖和活化的影响，并与环孢