关于酸味中药复方对糖尿病大鼠心肌组织晚期糖化终产物形成及其受体基因表达的影响.doc

关于酸味中药复方对糖尿病大鼠心肌组织晚期糖化终产物形成及其受体基因表达的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：关于酸味中药复方对糖尿病大鼠心肌组织晚期糖化终产物形成及其受体基因表达的影响 1 1 材料与方法 3 2 结果 5 3 讨论 5 文2：关于缬沙坦对糖尿病大鼠肾脏内皮素受体基因表达的影响 7 1 材料和方法 8 2 结 果 10 3 讨 论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 关于酸味中药复方对糖尿病大鼠心肌组织晚期糖化终产物形成及其受体基因表达的影响 文1：关于酸味中药复方对糖尿病大鼠心肌组织晚期糖化终产物形成及其受体基因表达的影响 Abstract：Objective To investigate the possible mechanism of Compounded Sour Medicinal Herbs (CSMH) in preventing and amelioration type 2 diabetic mellitus (T2DM) rats chronic complication， by analyzing the effects of CSMH on the deposition of advanced glycation end products (AGEs) and gene expression of their receptor myocardial tissue in T2DM rats. Methods The rats were injected with streptozotocin (STZ) through peritoneum， and fed with high-fat and high-caloric diets to induce T2DM animal models. Then the rats were randomly pided into three groups：untreated group (model group)， AG treated group and CSMH treated group. The deposition of AGEs and gene expression of RAGE in the myocardial tissue of T2DM rats were detected separately by fluorescence and real time PCR method. Results The deposition of AGEs and gene expression of their receptor in myocardial tissue in control group were lower than that of T2DM rats (P＜). After treated， the deposition of AGEs and gene expression of their receptor in myocardial tissue in CSMH treated group were decreased compared with that of untreated group (P＜)， and were equivalent to that of AG treated group. Conclusion CSMH can decrease the quantity of AGEs accumulation and down-regulated its receptor gene expression in the myocardial tissue in T2DM rats。 Key words：type 2 diabetic mellitus；myocardial tissue；AGEs；RAGE；gene expression；rat 糖尿病心肌病是以收缩和舒张功能障碍为特征的特异性心肌病，容易发生充血性心力衰竭[1-2]，临床尚无特效药物。动物实验证实，晚期糖化终产物(AGEs)在糖尿病心肌病的发生发展过程中扮演着重要角色，阻断其作用可减弱糖尿病所致的心肌组织结构损伤[3]。本研究以蛋白质非酶糖化终产物AGEs生成抑制剂氨基胍(AG)为对照，观察了酸味中药复方对2型糖尿病大鼠心肌组织AGEs形成及其受体(RAGE)mRNA表达的调节作用。 1 材料与方法 主要药物、试剂及饲料 酸味中药复方，由乌梅、吴茱萸、白芍