TPELISA法检测梅毒特异性抗体灰区内结果分析.docVIP

TPELISA法检测梅毒特异性抗体灰区内结果分析 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：TPELISA法检测梅毒特异性抗体灰区内结果分析 1 2 结果 3 3 讨论 4 文2：FQPCR检测ELISA法HBsAg弱反应性样本120例结果分析 6 1 资料与方法 6 2 结果 6 3 讨论 7 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 11 正文 TPELISA法检测梅毒特异性抗体灰区内结果分析 文1：TPELISA法检测梅毒特异性抗体灰区内结果分析 DOI：/-5547/.005 【Abstract】 Objective To analysis detection data in gray zone of treponema pallidum （TP） specific antibody by enzyme linked immunosorbent assay （ELISA）. Methods ELISA was applied to detect TP specific antibody in 15181 serum samples， and cutoff±20% as gray zone. In absorbance/cutoff （s/co） as ～， low value gray zone was ～ and high value gray zone was ～. Treponema pallidum particle assay （TPPA） was applied to confirm samples with gray zone optical delityin preliminary screening. Results There were totally 96 samples with value in gray zone among 15181 samples. Among the 96 cases， 59 cases were in low value gray zone and 37 cases in high value gray zone. There were 233 confirmed positive cases among 15181 cases， with positive rate as % （233/15181）， including 25 confirmed positive cases in gray zone， accounting for % （25/96） in gray zone and % （25/233） in all positive cases， and 71 confirmed negative cases， accounting for % （71/96） in gray zone. Reexamination by TPPA showed 25 positive cases in gray zone， with 8 cases in low value gray zone， accounting for % （8/25） in gray zone， and 17 cases in high value gray zone， accounting for % （17/25） in gray zone. Under TPPA dilution as 1∶80， 1∶160， 1∶320， 1∶640， 1∶1280， sample counts were respectively 3， 2， 1， 0， 2 in low value gray zone and 6， 4， 3， 2， 2 in high value gray zone. Conclusion In ELISA detection of TP antibody， positive and negative can not be singly determined by gray zone OD value and cutoff value， thus TPPA is necessary in reexamination。 【Key words】 Treponema pallidum； Specific antibody； Enzyme linked immunosorbent assay； Gray zone 梅毒是一类慢性接触性传染性疾病[1]， 人感染后可以导致全身多组织、器官的损伤和病变， 其病原微生物是TP。近年来， 梅毒在我国的发病呈上升趋势[2， 3]。目前，