β榄香烯对人胰腺癌Panc1细胞凋亡的影响.docVIP

β榄香烯对人胰腺癌Panc1细胞凋亡的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：β榄香烯对人胰腺癌Panc1细胞凋亡的影响 1 2 实验方法 3 3 统计学方法 6 4 结果 6 5 讨论 7 文2：β榄香烯对人胃癌BAC823细胞凋亡及P38MAPK磷酸化的影响 9 1 材料与仪器 10 2 方法 http: 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 β榄香烯对人胰腺癌Panc1细胞凋亡的影响 文1：β榄香烯对人胰腺癌Panc1细胞凋亡的影响 DOI：/-.018 Abstract： Objective To investigate the effects of β-Elemene on the apoptosis of human pancreatic cancer Panc-1 cells； To discuss its mechanism of action. Methods β-Elemene （10， 20， 40， 80， 160 μg/mL） were incubated to the Panc-1 cells in vitro cultured for 24 h， 48 h and 72h， and trypan blue refusal method was used to detect cell inhibition rate； Apoptosis rate was measured by TUNEL； Hoechst33258 fluorescent staining was used to observe the changes of the nucleus. The activity of Caspase-3， 8 and 9 were detected by ELISA. Western blot was used to detect the expressio of Fas， FasL and Cyt c and AIF. Results The activity of Panc-1 cells was obviously inhibited time/concentration dependent inhibition （P， P）， and the apoptosis rate increased after incubated with β-Elemene （P， P） after incubated with β-Elemene for 24 h， 48 h and 72 h； After giving β-Elemene 72 h， Panc-1 cells nucleus were broken obviously， and chromatin condeed and showed strong blue fluorescence， along with of apoptotic bodies； After incubated with β-Elemene for 48 h， Caspase-3， 8 and 9 activity significantly increased （P， P）； protein expressio of Fas， FasL， Cyt c and AIF were significantly enhanced （P， P， P）. Conclusion β-Elemene can inhibit Panc-1 cell proliferation， induce apoptosis， and the mechanism may be related to activating cell death receptor pathway and mitochondrial apoptosis pathway to play anti-tumor effects。 Key words： β-Elemene； pancreatic cancer； Panc-1 cells； cell apoptosis 虽然胰腺癌发病率仅占所有癌症的%，但其侵袭性强、预后差、死亡率高[1]。榄香烯是从姜科植物温郁金中提取的有效成分。β-榄香烯注射液是由我国自行研发的抗肿瘤药物，其抗肿瘤作用主要机制包括诱导肿瘤细胞凋亡、阻滞细胞周期及放化疗增敏作用、逆转肿瘤细胞多药耐药、抗肿瘤转移、提高机体免疫力等[2]。由于β-榄香烯具有抗肿瘤谱广、作用高效、毒副作用小等特点[3]，临床上已用于胶质瘤、乳腺癌、肝癌、卵巢癌、胃癌、大肠癌等恶性