与。。。一致的表达.pdfVIP

有关“一致”的表达 1. 然而，与Casola 等报道一致，顺次的BCR 刺激产生Fas 抗性，这样能保 护B 细胞免受Fas 诱导的细胞毒作用，有利于保证B 细胞应答的抗原特异性。 However, in keeping with the report by Casola et al. (12), sequential BCR stimulation produces Fas resistance and in so doing protects B cells from Fas-induced cytotoxicity, thereby promoting Ag specificity in B cell responses (14, 16, 17, 20). 2. 与之前实验结果一致，抗Ig 刺激B 细胞5 分钟之内出现大量的ERK1 和 ERK2 磷酸化。 Stimulation of naive B cells with anti-Ig produced substantial phosphorylation of ERK1 and ERK2 within 5 min, in keeping with previous work (36). 3. 与之前结果一致，抗Ig 刺激的初始B 细胞5min 内出现MEK 磷酸化。 Anti-Ig stimulation of naive B cells produced phosphorylation of MEK within 5 min, in keeping with previous work (39). 4. 与之前结果一致，抗Ig 刺激的初始B 细胞在5min 内诱发c-Raf 磷酸化， 用LY294002 抑制PI3K ，可阻断抗Ig 诱导的c-Raf 磷酸化。 Anti-Ig stimulation of naive B cells induced c-Raf phosphorylation within 5 min, in keeping with previous work (27), and PI3K inhibition with LY294002 inhibited anti-Ig-induced c-Raf phosphorylation. 5. 与之前结果一致，抗 Ig 刺激初始 B 细胞在 5min 内诱导 Ras 活化， LY294002 在一定程度上抑制BCR 诱导Ras 活化。 Anti-Ig stimulation of naive B cells induced Ras activation within 5 min, in keeping with previous work, and BCR-induced Ras activation was inhibited somewhat by LY294002 (36). 6. 与先前实验结果一致，该过程中仅ERK 和JNK 途径受影响，p38 MAPK 途径不受此影响，这表明p38 MAPK 途径的活化不同于ERK/JNK 的活化。 Both ERK and JNK were affected, whereas p38 MAPK was not, which fits well with previous work indicating that p38 MAPK activation is distinct from ERK/JNK activation (27, 52). 7. 与共培养所取得的结果一致，即MCF-7 细胞c-Jun 低组成性DNA 结合活 性与Mφ 共培养后增强了5 倍（图12，作为支持信息发表于PNAS 网站）。 Corresponding results were achieved in the coculture, where the low constitutive DNA binding activity of c-Jun in MCF-7 cells was up-regulated 5-fold by exposure to Mφ (Fig. 12, which is published as supporting information on the P