黄芪皂甙Ⅳ联合BMSCs移植对大鼠脑缺血再灌注海马神经细胞凋亡及L（职称论文资料）.docVIP

黄芪皂甙Ⅳ联合BMSCs移植对大鼠脑缺血再灌注海马神经细胞凋亡及L（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：黄芪皂甙Ⅳ联合BMSCs移植对大鼠脑缺血再灌注海马神经细胞凋亡及L 1 1 材料与方法 4 2 结 果 6 3 讨 论 7 文2：黄芪预处理对大鼠脑缺血再灌注损伤的保护作用 9 1 材料 10 2 方法和结果 10 3 讨论 12 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 黄芪皂甙Ⅳ联合BMSCs移植对大鼠脑缺血再灌注海马神经细胞凋亡及L（职称论文资料） 文1：黄芪皂甙Ⅳ联合BMSCs移植对大鼠脑缺血再灌注海马神经细胞凋亡及L Influence of Astragaloside ⅣCombined with Bone Mesenchymal Stem Cell Traplantation on Expressio of Livin，Caspase-9 and Neural Cell Apoptosis in Hippocampus after Cerebral Ischemia-reperfusion in Rats Wang Ying，Li Wenyuan，Yang Chunzhuang Department of Anatomy，Mudanjiang Medical College（Mudanjiang 157011） Abstract:Objective To investigate the effects of Astragaloside Ⅳ combined with bone mesenchymal stem cell（BMSCs） on the apoptosis of neural cells and expressio of livin and caspase-9 in hippocampal region after cerebral ischemia-reperfusion in A total of 42 healthy SD rats were randomly divided into sham operation group，model group，BMSCs group，and combination rat models with middle cerebral artery occlusion were models in BMSCs and combination groups were treated with suture method，and the rats in four groups were injected with PBS and PKH-26 labeled BMSCs suspeion via caudal vein 3 hou after middle cerebral artery occlusion（MCAO） Ⅳ（25 mg/kg） was administered in combination reperfusion 72h，neurological function scores were expressio of livin and caspase-9 protei were determined by immunohistochemical method and Western apoptosis of neural cells was detected by TUNEL labeled BMSCs were observed in with model and BMSCs group，the neurological scores in combination group were decreased significantly（P）.Compared with model group，the number of apoptotic neural cells and the expression in caspase-9 protei were significantly lower in BMSCs group and combination group（P）.The expression of livin protei was significantly increased（P）.The effect in combination group was more powerful than that in BMSCs group（P）.Conclusion The c