浅论Fis对伤寒沙门菌基因表达的系统调节作用（临床医学论文资料）.docVIP

浅论Fis对伤寒沙门菌基因表达的系统调节作用（临床医学论文资料） 文档信息 ： 文档作为关于“医学心理学”中“兽医”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文16272字，doc格式，可编辑。质优实惠，欢迎下载！ 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：浅论Fis对伤寒沙门菌基因表达的系统调节作用 2 1 材料与方法 3 2 结 果 7 3 讨 论 8 文2：浅论伤寒沙门菌基因组DNA芯片基因表达谱分析技术的建立与优化 10 1 材料和方法 12 2 结果 16 3 讨论 20 参考文摘引言： 21 原创性声明（模板） 22 文章致谢（模板） 23 正文 浅论Fis对伤寒沙门菌基因表达的系统调节作用（临床医学论文资料） 文1：浅论Fis对伤寒沙门菌基因表达的系统调节作用 [Abstract] Objective: To explore the systemic regulation of factor for inveion stimulation(fis) on gene expression in Salmonella enterica serovar ： The fis deleted mutant of serovar Typhi was prepared by homologous recombination mediated by suicide plasmid; Pulsefield gel electrophoresis was performed to investigate the genome structure of the fis mutant strain. The gene expression profiles of wildtype and fis deleted mutant of serovar Typhi incubated at log phase were investigated by serovar Typhi genomic DNA microarray analysis. A recombinant plasmid， pBADfis， containing the fis gene with its own promoter was cotructed. qRTPCR was performed to validate the results of microarray in some selected genes. The effect of Fis on the motility phenotype was established by tests on semisolid agar plates. Results： In the fis deleted mutant， the linear plasmid containing phase 2 flagellin gene fljBA was absent and the motility was also lost; gene expression profiles analysis revealed that expression of 94 genes were induced or decreased in the fis mutant at log phase; expression of the selected genes investigated by qRTPCR was similar to the result of microarray assay; after complementing fis gene in the fis deleted mutant， the motility and expression of selected genes were obviously restored. Conclusion： Fis may play the central role in coordinating the expression of genes involved in motile， and in invasion or substance metabolism in serovar Typhi。 [Key words] serovar Typhi; factor for inveion stimulation; microarray; PFGE; gene expression regulation 沙门