临床免疫学检验实验：ELISA Detect Hepatitis B Virus.pptVIP

ELISA Detect Hepatitis B Virus Conception of ELISA enzyme-linked immunosorbent assay: Enzyme-linked :enzyme labeled antibody or antigen Immuno: The antigen and antibody react with each other Sorbent: the reaction happens on the surface of solid phase carrier. The antigen antibody reaction on the surface of solid phase carrier, under the catalysis of enzyme, substance color will change though hydrolysis, oxidation or reduction reaction. According to the color, we can detect the concentration of antigen or antibody. Application: Antigen detection: double antibody sandwich method: suitable for protein or macromolecular antigen. competitive inhibition method: suitable for small molecules Antibody detection: double antigen sandwich method indirect method, competitive inhibition method capture method. What can we detect about HBV?? HBsAg has immunogenicity which is a symbol of HBV infection. HBsAb is a kind of protective antibody. Its appearance means body is in the recovery period or already has immunity. HBeAg is not only a symbol of HBV duplicate, but also an Infectious index. HBeAb and HBcAb are both indexes of infection that patient got in the past. If HBcAb-IgG appears which means body is infected now and virus begin to duplicate. method HBsAg: Double antibody sandwich method HBsAb: Double antigen sandwich method HBeAg: Double antibody sandwich method HBeAb: Neutral Competitive method HBcAb: Competitive inhibition method 1、Double antibody sandwich method detected antigen (two-steps method) coating antibody Sample Incubate wash Incubate wash E E E enzyme labelled Ab substance Incubate Stop buffer Sandwich forming Suitable for?protein and other?large molecules, such as HBsAg、HBeAg. E E E E E E 1、Double antibody sandwich method detected antigen (one-step method) coating antibody Sample Incubate wash E E E enzyme labelled Ab substance Incubate Stop buffer Sandwich