头孢他啶诱导大肠埃希菌内毒素释放及常用中药注射剂抗内毒素作用.docVIP

头孢他啶诱导大肠埃希菌内毒素释放及常用中药注射剂抗内毒素作用 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：头孢他啶诱导大肠埃希菌内毒素释放及常用中药注射剂抗内毒素作用 2 1 材料与方法 3 1.1 材料 3 1.2 方法［4］ 4 文2：头孢他啶诱导大肠埃希菌内毒素释放及常用中药注射剂抗内毒素作用的体外研究 5 1 材料与方法 6 1.1 材料 6 1.2 方法［4］ 7 2 结果 8 2.1 中药注射剂对头孢他啶MIC的影响 8 2.2 头孢他啶对大肠埃希菌内毒素释放的影响 8 2.3 中药注射剂对内毒素释放的影响 8 3 讨论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 头孢他啶诱导大肠埃希菌内毒素释放及常用中药注射剂抗内毒素作用 文1：头孢他啶诱导大肠埃希菌内毒素释放及常用中药注射剂抗内毒素作用 ABSTRACT Objective To study the facto that influence the endotoxin release induced by ceftazidime in different Escherichia coli concentratio, and to explore the antiendotoxin effects of 3 kinds of Chinese medicine injectio, namely Qingkailing injection, Shuanghuanglian injection and Xiyanping injection in vitro. Methods Twofold dilution to determine the MIC of ceftazidime, and determine if the MIC could be altered by 3 kinds of injectio when used in routine dosages; to study if ceftazidime (at 50×MIC) could affect the production of endotoxin while added to different concentratio of Escherichia coli which in logarithm phase; to determine the antiendotoxin activity of the three injectio when used concomitantly with ceftazidime. ResultsThe MIC of ceftazidime was 0.125 μg/ml, Qingkailing injection and Xiyanping injection had no effect on it, but Shuanghuanglian injection could lead it to increased; at high Escherichia coli concentratio (2.5×104～2.5×105 CFU/ml), ceftazidime induced a great deal of endotoxin release, but at low concentratio (2.5×10～2.5×103 CFU/ml), ceftazidime inhibited endotoxin release; the using sequence of Chinese medicine injectio and ceftazidime could affect endotoxin release. When Qingkailing injection and Xiyanping injection used after giving ceftazidime, they showed antiendotoxin effect, and Xiyanping injection was the better. Conclusion The endotoxin releasing activities of ceftazidime were not only related to the bacterial concentratio but also influenced by the sequence usage of Qinakailing or X