ClC3在糖尿病大鼠主动脉平滑肌肾脏及脑组织中的表达及其变化.doc

ClC3在糖尿病大鼠主动脉平滑肌肾脏及脑组织中的表达及其变化 目录 TOC \o 1-9 \h \z \u 目录 1 正文 2 文1：ClC3在糖尿病大鼠主动脉平滑肌肾脏及脑组织中的表达及其变化 2 1 材料与方法 3 1.1 实验材料和主要试剂 3 1.2 糖尿病大鼠模型制作 4 1.3 组织裂解与蛋白质的提取 4 1.4 Western Blot 4 1.5 统计分析 5 2 结 果 5 文2：ClC3在糖尿病大鼠主动脉平滑肌肾脏及脑组织中的表达及其变化 5 1 材料与方法 7 1.1 实验材料和主要试剂 7 1.2 糖尿病大鼠模型制作 7 1.3 组织裂解与蛋白质的提取 7 1.4 Western Blot 8 1.5 统计分析 8 2 结 果 8 2.1 ClC-3蛋白在大鼠主动脉平滑肌中的表达 8 2.2 ClC-3蛋白在大鼠肾脏皮质中的表达 9 2.3 ClC-3蛋白在大鼠肾脏髓质中的表达 9 2.4 ClC-3蛋白在大鼠脑组织中的表达 9 3 讨 论 9 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 ClC3在糖尿病大鼠主动脉平滑肌肾脏及脑组织中的表达及其变化 文1：ClC3在糖尿病大鼠主动脉平滑肌肾脏及脑组织中的表达及其变化 Abstract: 【Objective】 This study was designed to investigate the expression and changes of ClC-3 protein under condition of diabeticrats. 【Methods】 Diabetic rats were induced by an injection of streptozotocin. At the different periods of diabetic duration (2 weeks, 4 weeks, 8 weeks, 12 weeks, and 16 weeks), the expression and changes of ClC-3 protein in thoracic aortic smooth muscle, the kidneys (cortex, medulla) and the brai were detected using Western blotting. 【Results】 ClC-3 protein endogenously expressed in thoracic aortic smooth muscle, kidney (cortex, medullar) and brain of rat, and its molecular weight was between 80 ku and 110 ku. Comparing with the control group, the expression of ClC-3 was increased significantly in thoracic aortic smooth muscle and brain of diabetic rats from 8 weeks diabetic duration; however, it was decreased significantly in kidney medulla. There was no significant difference in expression of ClC-3 of kidney cortex between control and diabetic rats. 【Conclusion】 The endogenous expression of ClC-3 protein is altered in different tissues with the development of diabetes, and it is increased in thoracic aortic smooth muscle and brain, but it is decreased in kidney medulla. Key words: rats; aortic; kidney; brain; chloride channel protein 3 [J SUN Yat-sen Univ(Med Sci), 2007, 28（６）:613-616] 糖尿病患者易并发高血压