Cremophor-EL-DataSheet-生命科学试剂-MedChemExpress.pdf免费

Hotline: 400-820-3792 Inhibitors • Agonists • Screening Libraries www.MedChemE Cremophor EL Cat. No.: HY-Y1890 CAS No.: 61791-12-6 作⽤靶点: Others 作⽤通路: Others 储存⽅式: 4°C, protect from light * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) 溶解性数据 体外实验 H2O : 25 mg/mL (Need ultrasonic) 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存⽅式和期限。 BIOLOGICAL ACTIVITY ⽣物活性 Cremophor EL 是⼀种聚⼄氧基化表⾯活性剂。 体外研究 Cremophor EL is a widely used excipient. In the first 10 h, Cremophor EL at 5 mg/mL increases the impedance, then decreases it, and after 20 h of treatment, epithelial cell death is detected. Cremophor EL decreases cell viability and increases cell toxicity more than RH40. Both epithelial viability and monolayer integrity are seriously impaired at 10 to 50 mg/mL concentrations of Cremophor EL at 24-h treatment. Cremophor EL also enhances cell detachment [1]. PROTOCOL Cell Assay [1] Cell viability is determined by double fluorescent staining: bis-benzimide labels all cell nuclei, ethidium- homodimer-1 stains only dead cells. For morphological examinations, primary brain endothelial cells with tight barrier properties are used. Cells grown on glass coverslips are treated with Cremophor EL and RH40