人工感染呼肠孤病毒对拟穴青蟹部分免疫因子的影响.docxVIP

人工感染呼肠孤病毒对拟穴青蟹局部免疫因子的影响 邹清，2,李君华3,朱小明I （1.厦门大学环境与生态学院，福建厦门361005； 2.国家海洋局温州海洋环境监测中心站, 浙江 温州325000： 3.山东东方海洋科技股份，山东 烟台264003） ［摘要］采用注射和浸泡的方式人工感染拟穴青蟹呼肠孤病毒，研究了其对拟穴青蟹血细胞 密度以及血清中酚氧化前（PO）、超氧化物歧化酶（SOD）和碱性磷酸酶（AKP）活力的影 响?结果说明：注射（第II组）和浸泡（第IV组）方式均能感染健康育蟹，病发死亡时间为 7~9d,死亡率达10（）%,血细胞平均密度在试验36-72 h间迅速上升并达最高值，其血细胞 密度最高值分别为1.OO8XW7 cells/mL与L 243X107 cells/mL；感染病毒的青蟹PO活性均 大致呈现下降的趋势，其中，第IV组感染12 h青蟹PO活性相对对照组显著升高（3V0.05）, 其值为（6.90±1.54）,达实验所测PO活性最高值；各实验组血清SOD活性呈无规律的变 化；AKP活性感染组与对照组表现不同.说明测定血细胞密度以及PO和AKP活性可用来辅 助诊断青蟹疾病，而SOD活性变化不能很好地表征青蟹受病毒感染的状况. ［关键词］拟穴青蟹；呼肠孤病毒；免疫因了Effects of Artifical Infection Reovirus Trails on Some Immune Factors of Mud Crab,Scylla paramamosain ZOU Qing1,2, LI Jun-hua1,3, ZHU Xiao-niing1 (1.College of the Environment and Ecology,Xiamen University,Xiamen 361005,China;2.WenzhouMarine Environmental Monitoring Center of State Ocean Administration, Wenzhou325OOO,China； 3.Shandong Oriental Ocean Sci-Tech Co.,Ltd.,Yantai 264003,China) Abstract:The influence of artifical infection reovirous trails on the hemocyte density and the phenoloxidase(PO),superoxide dismutase (SOD) and alkaline phosphatase activity(AKP) of (he serum from Mud Crab,Scylla paramamosain was results showed that the healthy crab would be infected by virous injection and bath exposure in the virus ex(raction,There were no obvious difference between two treatments in mortality and latency period of the experimental animals,all crabs died in 7~9 d after es of some interrelated immune factors such as activities of PO, SOD and AKP displayed the downtrend day by day,while the hemocyte density rose evidently after 36 h and reached the peak value (2. 8534 X 107 cells/mL) at 72 h,It recovered to the same level as the control group after 108 h.In conclusion,the changes of hemocyte density and PO,AKP activity except SOD activity would be used to assist diagnoses of crab diseases. Key words:Scy//6? paramamosaim reovirus； immune factors