(7.5.27)--26.Three-dimensionalstructureoft建筑设备工程.pdfVIP

Molecular Cell, Vol. 15, 833–839, September 10, 2004, Copyright 2004 by Cell Press Three-Dimensional Structure Short Article of the Native Spliceosome by Cryo-Electron Microscopy Maia Azubel,1 Sharon G. Wolf,2 Joseph Sperling,3 between five major uridine-rich small nuclear RNP com- 1, and Ruth Sperling * plexes (U1, U2, U4, U5, and U6 snRNPs), as well as a 1Department of Genetics number of non-snRNP splicing factors, which are dy- The Hebrew University of Jerusalem namically recruited to the spliceosome when an exoge- Jerusalem 91904 nous pre-mRNA is added to a crude nuclear extract. Israel Density gradient centrifugation and native gel electro- 2 Department of Chemical Research Support phoresis revealed at least four intermediate complexes 3 Department of Organic Chemistry (E/CC, A, B, and C) in this assembly process. The U Weizmann Institute of Science snRNPs are the central components of the spliceosome. Rehovot 76100 They participate in splice site recognition and thus have Israel an essential function in splicing through cooperative RNA:RNA interactions between the snRNAs and with the pre-mRNA (Brow, 2002; Staley and Guthrie, 1998). Summar