实时荧光定量PCR检测HBVDNA测量不确定度的评定(1).pdfVIP

实时荧光定量PCR检测HBV-DNA测量不确定度的评定 孔小祥;仲崇明 【摘要】Objective To investigate the uncertainty in measurement of HBV- DNA by real-time fluorescence quantitative PCR and evaluate its clinical application. Methods Referring to CNAS-TRL-001:2012medical laboratory- Evaluation and ex-pression of uncertainty in measurement,the data of internal quality control was used to assess measurement reproducibility and CRM was used to assess measurement bias. The two variables of reproducibility and bias were combined to calculate the uncertain-ty in measurement. Results Expanded uncertainty of high value,med