90plus 纳米粒度仪应用案例.doc

90plus 纳米粒度仪应用案例-21 文献名：Cytosolic Delivery of Membrane-Impermeable Molecules in Dendritic Cells Using pH-Responsive Core?Shell Nanoparticles 作者： Yuhua Hu, Tamara Litwin, Arpun R. Nagaraja, Brandon Kwong, Joshua Katz, Nicki Watson, Darrell J. Irvine Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, and Whitehead Institute, 9 Cambridge Center, Cambridge, Massachusetts 02142 摘要：Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the “proton sponge” effect. To exploit this mechanism, we created nanoparticles with a segregated core?shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticles were synthesized with a pH-responsive core and hydrophilic charged shell designed to disrupt endosomes and mediate drug/cell binding, respectively. By sequestering the relatively hydrophobic pH-responsive core component within a more hydrophilic pH-insensitive shell, nontoxic delivery of small molecules and proteins to the cytosol was achieved in dendritic cells, a key cell type of interest in the context of vaccines and immunotherapy.