共聚焦细胞课.pptxVIP

共聚焦细胞课;高级显微镜原理 正置、倒置显微镜 细胞遗传工作站 活细胞工作站 激光显微分离系统 激光共聚焦显微镜;概述;Conventional fluorescence microscope Confocal microscope ;光镜2um;历史;Confocal microscopy comes of age JG White WB Amos. Nature 328, 183 - 184 (09 July 1987) Zeiss、Leica、Olympus、Nikon;Zeiss LSM510 激光扫描共聚焦显微镜;Zeiss LSM510 META 激光扫描共聚焦显微镜;Zeiss LSM510 META 激光扫描共聚焦显微镜;Nikon A1R 激光扫描共聚焦显微镜;Prairie Ultima IV 活体双光子显微镜;国家光电实验室（武汉）自制随机定位双光子显微镜;Leica TCS SP5 激光共聚焦扫描显微镜;基本原理;Two ways to obtain contrast in light microscopy. The stained portions of the cell in (A) reduce the amplitude of light waves of particular wavel