超微铁皮石斛对脾虚便秘小鼠肠道微生物的影响研究.docx

超微铁皮石斛对脾虚便秘小鼠肠道微生物的影响研究 摘要： 本文研究了超微铁皮石斛对脾虚便秘小鼠肠道微生物的影响。通过建立小鼠脾虚便秘模型，将小鼠随机分为四组，分别为对照组、超微铁皮石斛组、枸杞组、超微铁皮石斛+枸杞组，观察各组小鼠的粪便性状、肠道微生物以及肠黏膜形态，并进行相关分析和统计。 结果表明，超微铁皮石斛可以明显改善小鼠的脾虚便秘症状，提高粪便水分含量，缓解便秘。同时，超微铁皮石斛可以促进益生菌（如Bifidobacterium）的生长，降低致病菌（如Staphylococcus aureus和Escherichia coli）的含量，从而改善肠道微生态平衡。此外，超微铁皮石斛还可以促进肠黏膜细胞的增殖和分化，增强肠道屏障功能，降低致炎反应。 综上所述，超微铁皮石斛在治疗脾虚便秘方面具有潜在的效果，并且可以通过调节肠道微生物和维护肠黏膜健康来发挥作用，值得进一步研究和应用。 关键词：超微铁皮石斛；脾虚便秘；小鼠；肠道微生物；肠黏膜 Abstract： This study investigated the effect of ultrafine dendrobium on gut microbiota in mice with spleen deficiency constipation. A mouse model of spleen deficiency constipation was established, and the mice were randomly divided into four groups: control group, ultrafine dendrobium group, goji berry group, and ultrafine dendrobium + goji berry group. Fecal characteristics, gut microbiota, and intestinal mucosal morphology were observed and analyzed between the groups. Results showed that ultrafine dendrobium significantly improved the symptoms of spleen deficiency constipation in mice, increased fecal moisture content and relieved constipation. Furthermore, ultrafine dendrobium promoted the growth of beneficial bacteria (such as Bifidobacterium), and reduced the abundance of pathogenic bacteria (such as Staphylococcus aureus and Escherichia coli), thus modulating gut microbial ecology. In addition, ultrafine dendrobium promoted the proliferation and differentiation of intestinal mucosal cells, enhanced the intestinal barrier function, and reduced the inflammation response. In summary, ultrafine dendrobium has potential therapeutic effects on spleen deficiency constipation, which is attributed to its effects on gut microbiota and gut mucosal integrity. Thus, further studies on the application of ultrafine dendrobium as a potential treatment are warranted. Keywords: ultrafine dendrobium; spleen deficiency constipation; mice; gut microbiota; gut mucosa