脐带血基础免疫物质分析.docVIP

脐带血基础免疫物质分析 ???? 【摘要】??　　目的：?通过胎儿脐带血免疫状况的研究，为脐带血有形和无形成分在免疫方面的应用提供理论依据。方法：?应用血液常规检验、试剂盒以及液体闪烁计数仪和流式细胞仪对白细胞分类、免疫球蛋白、细胞因子、脐血T细胞抗原表面标志和脐血有核细胞NK活性进行测定。结果：?与成人外周血中免疫成分相比，胎儿脐带血中IgA和IgE含量明显减少，IgG无明显改变；脐血浆中GCSF和GMCSFCSF和GMCSF等相关造血因子和原始的造血干细胞成分，虽然淋巴细胞的发育多处于幼稚期，但大量相关的免疫细胞和细胞因子均可作为免疫治疗的基础物质。 【关键词】??细胞因子类?免疫球蛋白类?血液?脐带 　　［Abstract］?Objective:?To?study?the?immunity?state?of?foetus?cord?blood,?so?as?to?provide?theorty?basis?for?the?application?of?immunological?substances?from?foetus?cord?blood?in?hematoplasma?immunotherapy.?Methods：?Using?blood?routine?test?kits,?liquid?scintillation?counter?and?flow?cytometry?to?detect?leukocyte?classfication,?immune?globulin,?T?cell?antigen?surface?marker?and?NK?cell?respectively.?Results：IgA?and?IgE?in?cord?blood?were?much?less?than?in?adult?peripheral?blood,?but?IgG?was?nearly?the?same?as?that?in?adult?peripheral.?GCSF?and?GMCSF?in?cord?blood?plasma?were?11.37?and?15.81?times?respectively?of?those?in?adult?peripheral?blood,while?tumor?necrosis?factor?(TNF)?was?a?little?bit?lower,?and?heterophil?granulocyte?level?was?close?to?the?lower?limit?in?adult?peripheral?blood.?Metagranulocyte?and?myelocyte?levels?were?obviously?lower?than?those?in?adult?peripheral?blood.Leukomonocyte?level?was?about?the?same?as?that?of?adult.The?counts?of?subpopulations?CD3+,CD4+,CD8+?of?T?cell?in?cord?blood?were?significantly?lower?than?those?in?adult?peripheral?blood?while?those?of?CD34+?and?CD45RA?were?higher.NK?cell?killing?activity?was?slightly?lower?than?that?of?adult?blood.?Conclusion：?Relative?hemopoiesis?factors?and?native?hematopoietic?stem?cell?components,?GCSF?and?GMCSF,??are?abundant?in?cord?blood?and?can?be?widely?used?in?clinical?immunotherapy,?even?though?most?T?lymphocytes?in?cord?blood?are?in?naive?stages?of?development.　　　［Key?words］?tissue?factor;?cytokines；immunoglobulins；blood；umbilical?cord???????　　脐血中的有形和无形成分与脐血的免疫活性密切相关，各种活性因子的存在对不同脐血细胞的发育和功能起重要的调节作用[1～3]。这些活性因子和免疫细胞既与脐血造血干细胞或祖细胞的成功移植密切相关,也与脐血中的免疫成分在抗肿瘤中的广泛应用有关。有关脐血中的有形和无形免疫活性成分的检测国内报