分子生物学许晓东.pptVIP

In DNA polymerase, the nucleotide binding pocket is too small to allow the presence of a 2’OH on the incoming nucleotide. This space is occupied by two amino acids (discriminator amino acids) that make van der Waals contacts with the sugar ring. 当前第94页\共有149页\编于星期五\3点 DNA Polymerase Holoenzyme Consists of Subcomplexes A clamp loader places the processivity subunits on DNA, where they form a circular clamp around the nucleic acid. One catalytic core is associated with each template strand. DNA polymerase III holoenzyme assembles in stages, generating an enzyme complex that synthesizes the DN