DB37_T 3801-2019花生品种纯度鉴定技术规程—SSR标记法.pdf

ICS65. 020. 0190 8DB37山東东省地電方标准DB37/T3801—2019花生品种纯度鉴定技术规程一SSR标记法Protocol of purity identification for peanut variety usingSSR markers2019-12-24发布2020-01-24实施山东省市场监督管理局发布 DB37/T 3801—2019目次前言11范围2规范性引用文件3术语和定义4原理5仪器设备6试剂，溶液配制8引物信息操作步骤9. 1样品准备9. 2单粒种子的DNA提取9. 3PCR 扩增9. 4PCR产物检测10判定方法与原则10. 1判定方法10.2判定原则附录A（规范性附录）溶液配制附录B（资料性附录）推荐引物附录C（资料性附录）参照品种名单， DB37/T 3801—2019表B.1推荐引物（续）序号引物连锁群引物序列(5→3°)常见等位变异bp参照品种56 zh. h052556/71zh. h5327626604*212 GNB556A01Ftcagggtgggttaccaacat68zh. h2413Rtaatccacattgaaccgacg71zh. h241077zh. h086880zh. h223198zh. h1331 64zh. h503474zh. h47498Lzh, h097713PM308未定位FccttettetttctcctcctcaRcaattcgtgatagtattttattggaca80zh. h276482zh. h246486zh. h240592zh. h0680986zh, h2456403zh. h276414pPGPseq2C11403Ftgacetcaattttggggaag418zh. h4749Rgccactattcatcgcggta439zh. h2405450zh. h2406461zh. h0436180zh, h4779180/192zh. h5327192zh. h240615pPGSseq19D6B05FtttgttatgctcacaccccaRaaaaatgaagcaatattttgttgttag192/204zh. h2152200zh. h2508204zh, h0537210zh. h50348 DB37/T 3801—2019CC附（资料性附录）参照品种名单C. 1参照品种名单见表C.1表C.1参照品种名单序号种质库统一编号品种名称序号种质库统一编号品种名称1zh. h0099籁榆小站秩38xzh. h2292浒山半旱种zh. h0102启东赤豆花生39zh. h2330全州凤凰花生3zh. h0125涡阳站秧40 zh. h2384英德白沙鸡豆仔4zh. h0436蒲庙花生41zh. h2405试花1号5zh. h0477睦屋技豆42 xzh. h2406试花3号6zh. h0525锦交1号43 zh. h2410博山站秋子zh. h0531腾县腾子花生44 xzh. h2413沂南小麻叶8zh. h0537巨野小花生45zh. h2425嘉祥长秧zh. h0540栖霞爬蔓小花生46 zh. h2426沂南大铺秩10zh. h0678托克逊小花生47zh. h2456郑72511zh. h0680北京大粒墩48zh. h2461长垣一把抓12 zh. h0764昆谐大粒墩49 zh. h2462阳83713zh. h0861反修1号50 zh. h2464濮阳二楼14 zh. h0868栖霞半棘皮zh. h2466王屋花生15zh. h0930莱芜蔓 52 zh. h2477开封大接秩1666044x夏津小二秋Szh. h2499南江大花生17 zh. h0949牟平大粒蔓54 xzh. h2508南溪二郎子18zh. h0977汶上蔓生55zh. h2550霸王鞭19 zh. h1315发财生56 zh. h2561潜山大果20zh. h1331柳城等豆57xzh. h2562宿松土花生21北镇大花生8Szh. h2591邵东中扯子22 zh. h1395花3359 xzh. h2608大埔种23zh. h1585洪洞花生60zh. h2682岑巩膜花生24 zh. h1602辽中四鞍红19zh. h2702锦交四号25zh. h1672临县花生62zh. h2764早熟红粒26zh. h1689青川小花生9zh. h4673肺归磨坪红皮花生27zh. h1797鄂花5号64zh. h4749赣花232628zh. h1816金寨蔓生-80选65zh. h4779汕油52329 zh. h1843茶陵打子66zh. h4923桑植C30 zh. h1961舞油红4号67zh. h5030小河大花生