精选抗体抗体的多样性及其产生机制讲义.pptVIP

当前第63页\共有103页\编于星期一\10点 In heavy chains, the V, D and J segments encode the variable domain while the C segment encodes the constant domain. In light chains, the V and J segments encode the variable domain while the C segment encodes the constant domain. 当前第64页\共有103页\编于星期一\10点 抗体基因的重排 当前第65页\共有103页\编于星期一\10点 During B cell maturation, immunoglobulin genes undergo rearrangement. 当前第66页\共有103页\编于星期一\10点 During B cell maturation, immunoglobulin genes undergo rearrangement. 当前第67页\共有103页\编于星期一\10点 During B cell maturation, immunoglobulin genes undergo rearrangement. 当前第68页\共有103页\编于星期一\10点 During B cell maturation, immunoglobulin genes undergo rearrangement. 当前第69页\共有103页\编于星期一\10点 The kappa and lambda loci undergo similar rearrangement. Since there are no D segments, there is a single V--J rearrangement. In both heavy and light chain rearrangement, a number of J segments may remain between the V(D) and C segments. These are included in the RNA transcript but are spliced out during RNA processing. The final light chain mRNA contains one VJC unit. Kuby Figure 5-4 当前第70页\共有103页\编于星期一\10点 Kuby Figure 5-4 当前第71页\共有103页\编于星期一\10点 当前第72页\共有103页\编于星期一\10点 抗体基因重排的机制 当前第73页\共有103页\编于星期一\10点 （1）重组信号序列 (recombination signal sequence, RSS) 在VH和VL基因3’端、J基因5’端和D基因两端有高度保守的核苷酸序列，包括一个7核苷酸组成的七聚体(heptamer)和一个9核苷酸组成的九聚体(nonamer)，两者间隔12?1或23?1个核苷酸，称为RSS。 当前第74页\共有103页\编于星期一\10点 只有间隔12个碱基对和间隔23个碱基对的RSS之间发生重组，而间隔长度相等的RSS之间不发生重组，这就是12/23碱基对规律(12/23 base pair rule)，也叫做1+2圈规律(1+2 turn rule)。 当前第75页\共有103页\编于星期一\10点 Kuby Figure 5-3 当前第76页\共有103页\编于星期一\10点 Recombination signal sequences - conserved sequences in regions just upstream or downstream of gene segments. Consist of a conserved heptamer and nonamer with a 12 or 23 bp spacer. Kuby Figure 5-3 当前第77页\共有103页\编于星期一\10点 The one-turn/two-turn rule (12/23 rule) - recombination occurs only between a segment with a 12 bp spacer and a segment with a 23 bp spacer. Recombination Signal Sequences Direct Recombination 当前第78页\共有103页\编于星期一\10点 Recom