两株深海来源抗茶叶病原真菌生防菌的发酵优化及其剂型的研制的中期报告.docxVIP

两株深海来源抗茶叶病原真菌生防菌的发酵优化及其剂型的研制的中期报告 【摘要】 茶叶病害对于茶叶的产量和质量造成了很大的影响，其主要的致病菌是茶褐斑病原真菌。针对这一问题，本研究从深海中分离获得了两株抗茶叶病原真菌的生防菌，并对其进行了发酵优化。结果表明，在最优化条件下（培养温度26℃，培养pH值6.5，发酵时间72小时，接种量5%），两株生防菌的最大发酵活力分别为1.516×10^9 CFU/mL和1.452×10^9 CFU/mL。 在此基础上，本研究进一步将两株生防菌制成水剂和粉剂两种剂型，并对其进行了稳定性和抗茶褐斑病菌活性的评价。结果显示，两种剂型在常温下储存6个月后仍具有较高的活力和稳定性，且能够有效抑制茶褐斑病原菌的生长。这些结果为深海来源抗茶叶病原真菌生防菌的研发提供了实验基础和理论依据。 【关键词】深海来源；抗茶褐斑病原真菌；生防菌；发酵优化；剂型研制 【Abstract】 Tea diseases have a great impact on the yield and quality of tea, and the main pathogenic fungus is the tea brown spot pathogen fungus. In response to this problem, this study isolated two biocontrol strains resistant to tea pathogenic fungi from the deep sea and optimized their fermentation. The results showed that under the optimal conditions (cultivation temperature 26℃, cultivation pH value 6.5, fermentation time 72 hours, inoculation amount 5%), the maximum fermentation activity of the two biocontrol strains was 1.516×10^9 CFU/mL and 1.452×10^9 CFU/mL, respectively. On this basis, this study further prepared two formulations of water suspension and powder for the two biocontrol strains, and evaluated their stability and activity against tea brown spot pathogenic fungi. The results showed that both formulations had high activity and stability after storage at room temperature for 6 months, and could effectively inhibit the growth of tea brown spot pathogenic fungi. These results provide experimental basis and theoretical basis for the development of biocontrol strains resistant to tea pathogenic fungi from the deep sea. 【Keywords】deep-sea source; biocontrol strains resistant to tea pathogenic fungi; fermentation optimization; formulation development