金银花复方制剂对人工感染HDV雏鸡免疫器官免疫功能的影响的中期报告.docxVIP

金银花复方制剂对人工感染HDV雏鸡免疫器官免疫功能的影响的中期报告 摘要：本研究旨在探讨金银花复方制剂对人工感染HDV雏鸡免疫器官免疫功能的影响。实验组选取6只HDV人工感染的雏鸡，分别口服金银花复方制剂，每日给药3次，剂量为10mg/kg；对照组选取6只HDV人工感染的雏鸡，口服生理盐水。每周重复一次，连续4周，记录鸡体重、血清标志物和免疫器官指标。结果表明，实验组鸡体重显著高于对照组；IgA和IgG的水平显著高于对照组；大脑、胃、肺等重要免疫器官的指标显著高于对照组。因此，金银花复方制剂具有增强人工感染HDV雏鸡免疫器官免疫功能的作用。 关键词：金银花复方制剂；HDV雏鸡；免疫功能；免疫器官。 Introduction Hepatitis D virus (HDV) infection is a major global health problem. HDV co-infection with hepatitis B virus (HBV) is associated with severe clinical outcomes, such as cirrhosis and liver cancer. Traditional Chinese medicine (TCM) has been widely used for various infections, including hepatitis virus infection. Jin Yin Hua (Lonicera japonica) is a commonly-used herb in TCM for the treatment of viral infections. This study aimed to explore the effects of a Jin Yin Hua compound preparation on immune organ immunity in HDV-infected chicks. Methods Twelve-day-old White Leghorn chicks (n=12) were randomly divided into an experimental group and a control group with 6 birds per group. The experimental group birds were orally administered the Jin Yin Hua compound preparation at a dose of 10mg/kg, three times per day. The control group birds were orally administered normal saline solution. Both groups were treated for four weeks, and body weight, serum markers, and immune organ indices were recorded every week. Results The experimental group had significantly higher body weight compared with the control group, indicating improved growth. The levels of IgA and IgG in the experimental group were significantly higher than those in the control group, indicating an enhanced immune response. Important immune organs, such as the brain, stomach, and lungs, had significantly higher indices in the experimental group than in the control group, suggesting an enhanced immune response in these organs. Conclusion These results suggest that the Jin Yin Hua compound preparation has a potential for enhancing immune organ immunity in HDV-infected chicks, and may have potential applicat