β2-上腺素受体介导雌激素对大鼠心肌细胞缺氧复氧损伤保护作用的研究的中期报告.docxVIP

β2-上腺素受体介导雌激素对大鼠心肌细胞缺氧复氧损伤保护作用的研究的中期报告 摘要： 本研究旨在探究β2-上腺素受体（β2-AR）介导雌激素对大鼠心肌细胞缺氧复氧损伤的保护作用。采用体外模型，利用大鼠心肌细胞，将细胞分为对照组、缺氧组、复氧组、β2-AR拮抗组、雌激素组和β2-AR拮抗+雌激素组。通过MTT法、LDH释放率和ROS水平的检测，研究了不同组细胞的生存率、损伤程度和氧化应激水平。结果表明，缺氧后细胞的存活率和LDH释放率显著降低，ROS水平明显升高。而雌激素和β2-AR拮抗剂组分别能够降低LDH释放率和ROS水平，提高细胞存活率。此外，β2-AR拮抗和雌激素联合使用后，细胞的保护效果更好。综上所述，本研究结果显示β2-AR介导的雌激素具有脂质过氧化、线粒体功能和细胞死亡等因素上的保护作用，对缺氧复氧损伤的大鼠心肌细胞有一定的保护作用。 关键词：β2-上腺素受体；雌激素；缺氧复氧；大鼠心肌细胞；细胞保护 Abstract: The present study aimed to investigate the protective effect of estrogen mediated by β2-adrenergic receptor (β2-AR) on rat myocardial cell damage under hypoxia-reoxygenation conditions. In the in vitro model, rat myocardial cells were divided into control group, hypoxia group, reoxygenation group, β2-AR antagonist group, estrogen group and β2-AR antagonist + estrogen group. The survival rate, damage degree and oxidative stress level of cells in different groups were measured by MTT assay, LDH release rate and ROS level. The results showed that the survival rate and LDH release rate of cells were significantly reduced and the ROS level was significantly increased after hypoxia. Estrogen and β2-AR antagonists could reduce the LDH release rate and ROS level and increase the cell survival rate. In addition, the combined use of β2-AR antagonists and estrogen showed a better protective effect on cells. In conclusion, the results of this study showed that β2-AR-mediated estrogen has a protective effect on factors such as lipid peroxidation, mitochondrial function and cell death, and has a certain protective effect on rat myocardial cells under hypoxia-reoxygenation injury. Keywords: β2-adrenergic receptor; estrogen; hypoxia-reoxygenation; rat myocardial cells; cell protection