调控Nrf2-ARE通路对小鼠脊髓损伤治疗作用的实验研究的中期报告.docxVIP

调控Nrf2-ARE通路对小鼠脊髓损伤治疗作用的实验研究的中期报告 摘要 本文介绍了一项实验研究，旨在探讨Nrf2-ARE通路调控对小鼠脊髓损伤治疗的作用。实验使用40只C57BL/6小鼠，随机分为4组。其中，对照组（n=10）没有接受任何治疗，模型组（n=10）施加T10脊髓损伤模型并仅接受生理盐水治疗，治疗组A（n=10）施加T10脊髓损伤模型并接受紫杉醇治疗，治疗组B（n=10）施加T10脊髓损伤模型并接受紫杉醇加NAC（N-乙酰半胱氨酸）治疗。实验从术后第1天开始每天给予不同治疗。实验结果表明，模型组小鼠的组织学损害明显大于对照组，而治疗组A和治疗组B的组织学损害较轻，其中治疗组B的效果最好。此外，与模型组相比，治疗组A和治疗组B的Nrf2、HO-1、NQO1和GCLC的蛋白表达量均有所上升，且治疗组B的效果最好。综上所述，本实验结果表明，通过调控Nrf2-ARE通路可以减轻小鼠脊髓损伤的损害，对治疗脊髓损伤具有潜在的临床价值。 关键词：Nrf2-ARE通路，小鼠，脊髓损伤，紫杉醇，NAC Abstract This paper introduces an experimental study to explore the role of Nrf2-ARE pathway regulation in the treatment of spinal cord injury in mice. 40 C57BL/6 mice were randomly divided into four groups. The control group (n=10) did not receive any treatment, the model group (n=10) received only saline treatment after applying T10 spinal cord injury model, treatment group A (n=10) received paclitaxel treatment after applying T10 spinal cord injury model, and treatment group B (n=10) received paclitaxel and NAC treatment after applying T10 spinal cord injury model. The treatment was given daily from the 1st day after the operation. The results showed that the histological damage of the model group was significantly greater than that of the control group, while the histological damage of treatment group A and treatment group B was lighter, and treatment group B had the best effect. In addition, compared with the model group, the protein expression levels of Nrf2, HO-1, NQO1 and GCLC in treatment group A and treatment group B all increased, and treatment group B had the best effect. In conclusion, the results of this experiment show that by regulating the Nrf2-ARE pathway, the damage of spinal cord injury in mice can be reduced, and it has potential clinical value for the treatment of spinal cord injury. Keywords: Nrf2-ARE pathway, mice, spinal cord injury, paclitaxel, NAC