欧美杨X藏川杨杂交群体构建及其苗期相关性状QTLs初步分析的中期报告.docxVIP

欧美杨X藏川杨杂交群体构建及其苗期相关性状QTLs初步分析的中期报告 摘要： 本文从杨树（Populus）群体基因组水平构建欧美杨X藏川杨杂交群体，分析苗期相关性状和QTLs，为后续选种和育种提供理论基础。结果显示，杂交后代平均植株高、地径、基径等指标均高于亲本，说明杂交具有显著优势。同时，通过RAD-seq技术获得26,285个SNP标记，用于群体基因型分析和QTLs定位。在11个苗期相关性状中，共检测到266个QTLs，其中树高、基径、叶面积、叶绿素等指标中的QTLs数目较多。这些QTLs分布在杨树基因组的不同染色体上，部分QTLs可以解释不同性状之间的相关性。本文研究为后续的杨树育种和基因组学研究提供了重要的理论和实验基础。 关键词：欧美杨、藏川杨、杂交群体、苗期相关性状、QTLs定位 Abstract： In this study, we constructed a hybrid population of European and American poplar (Populus) X Tibetan poplar (Populus) at the genome level, and analyzed the QTLs related to seedling traits, providing a theoretical basis for subsequent breeding and genetics. The results showed that the hybrid offspring had significantly higher plant height, ground diameter, and basal diameter than the parents, indicating that hybridization had significant advantages. At the same time, 26,285 SNP markers were obtained by RAD-seq technology for population genotype analysis and QTLs mapping. A total of 266 QTLs were detected among 11 seedling-related traits, among which there were more QTLs in tree height, basal diameter, leaf area, chlorophyll and other indicators. These QTLs were distributed on different chromosomes of the Populus genome, and some QTLs could explain the correlation between different traits. This study provides important theoretical and experimental basis for subsequent Populus breeding and genetics research. Keywords: European and American poplar, Tibetan poplar, hybrid population, seedling traits, QTL mapping.