刺苦草根状茎酶促褐变机理研究的中期报告.docxVIP

刺苦草根状茎酶促褐变机理研究的中期报告 【摘要】 刺苦草（Lithospermum erythrorhizon Sieb. et Zucc.）是一种重要的药用植物，其根状茎中含有丰富的紫草素，被广泛应用于医药、化妆品等领域。然而，刺苦草根状茎在加工和贮藏过程中容易发生褐变现象，影响产品品质和经济效益。本研究探究了刺苦草根状茎褐变的机理，采用高通量测序技术对褐变和未褐变根状茎的基因表达进行比较分析，发现差异表达基因涉及生物合成、代谢调节等多个通路。进一步筛选出与褐变相关的基因，其中最具代表性的包括β-葡萄糖苷酶、过氧化物酶、木质素单加氧酶等，这些酶参与了酚类物质的降解和氧化反应。通过酶活性测定和qRT-PCR验证，证实这些酶与刺苦草根状茎褐变密切相关。本研究为深入理解刺苦草根状茎褐变的分子机理提供了新的线索，为优化产品加工和贮藏提供了理论基础。 【关键词】 刺苦草；根状茎；褐变；基因表达；酶活性；qRT-PCR 【Abstract】Lithospermum erythrorhizon Sieb. et Zucc. is an important medicinal plant, and its root tuber contain abundant shikonin, which is widely used in the fields of medicine, cosmetics, etc. However, the root tubers of L. erythrorhizon are prone to browning during processing and storage, which affects the product quality and economic benefits. In this study, we explored the mechanism of browning in L. erythrorhizon root tubers, and conducted comparative analysis of gene expression between browned and non-browned root tubers using high-throughput sequencing technology. Our results showed that differentially expressed genes were involved in multiple pathways including biosynthesis and metabolic regulation. Furthermore, we screened out genes related to browning, among which the most representative enzymes included β-glucosidase, peroxidase, lignin monooxygenase, etc., which were involved in the degradation and oxidation of phenolic compounds. Enzyme activity assays and qRT-PCR verification confirmed that these enzymes were closely related to browning of L. erythrorhizon root tubers. This study provides new clues for understanding the molecular mechanism of browning in L. erythrorhizon root tubers, and provides a theoretical basis for optimizing product processing and storage. 【Keywords】L. erythrorhizon; root tuber; browning; gene expression; enzyme activity; qRT-PCR