锌指基因ZNF580过表达对大鼠颈动脉球囊损伤修复作用及其机制的研究的中期报告.docxVIP

锌指基因ZNF580过表达对大鼠颈动脉球囊损伤修复作用及其机制的研究的中期报告 摘要：本研究旨在探讨锌指基因ZNF580在大鼠颈动脉球囊损伤修复中的作用及其机制。选取SPF级健康雄性SD大鼠，进行颈动脉球囊损伤模型制备，随机分为模型组和ZNF580干扰组。经过常规观察，发现ZNF580过表达能够减轻大鼠颈动脉球囊损伤程度，降低动脉内皮细胞凋亡率和MMP2、MMP9表达水平。同时，ZNF580过表达还能够促进血管内皮细胞增殖和血管再生，提高皮肤血流量和局部组织氧合水平。实验结果表明，ZNF580过表达在大鼠颈动脉球囊损伤修复中发挥重要作用，其机制可能与其抑制内皮细胞凋亡和降低MMP2、MMP9表达水平有关。 关键词：锌指基因ZNF580，颈动脉球囊损伤，内皮细胞凋亡，MMP2，MMP9 Abstract: This study aims to explore the role and mechanism of zinc finger gene ZNF580 in repairing carotid artery balloon injury in rats. SPF-level healthy male SD rats were selected, and carotid artery balloon injury model was prepared and randomly divided into model group and ZNF580 interference group. After routine observation, it was found that ZNF580 overexpression could alleviate the degree of carotid artery balloon injury in rats, reduce the apoptosis rate of endothelial cells and the expression levels of MMP2 and MMP9. At the same time, ZNF580 overexpression can also promote the proliferation of vascular endothelial cells and vascular regeneration, increase the skin blood flow and the local tissue oxygenation level. The experimental results show that ZNF580 overexpression plays an important role in repairing carotid artery balloon injury in rats, and its mechanism may be related to its inhibition of endothelial cell apoptosis and reduction of MMP2 and MMP9 expression levels. Keywords: zinc finger gene ZNF580, carotid artery balloon injury, endothelial cell apoptosis, MMP2, MMP9.