软式内镜清洗刷对生物膜清除效果的调查与实验研究的中期报告.docxVIP

软式内镜清洗刷对生物膜清除效果的调查与实验研究的中期报告 摘要：软式内镜用于医学检查已成为现代医学领域的重要技术之一，但如果不及时清洗消毒就会积累生物膜，从而可能导致交叉感染或微生物污染。本研究旨在评估软式内镜清洗刷对生物膜清除效果的影响，采用微生物分离培养和扫描电镜观察结合的方法。我们收集了医院内多个抗生素耐药菌株和标准菌株，确定细菌和真菌的生长条件并制备生物膜模型。然后使用不同类型和形状的软式内镜清洗刷进行清洗，并对刷头的材料、形状、旋转速度等要素进行比较。我们的结果显示，无论使用何种类型和形状的清洗刷，都能够有效清除生物膜，但在材料和形状方面的细微差异可以影响清洗效果。具体来说，硬度较高的刷头材料和球形刷头形状表现出更好的生物膜清除效果，高速旋转也有利于清除生物膜。课题的后续工作将对高效清洗软式内镜的最佳方式进行更深入的研究和测试。 关键词：软式内镜；生物膜；清洗刷；清洗效果；微生物污染 Introduction: Soft endoscopes are widely used in medical practice. However, if they are not cleaned and disinfected in a timely manner, biofilms may accumulate, leading to cross-infection or microbial contamination. Therefore, it is important to assess the effectiveness of cleaning brushes in removing biofilms. Methods: Multiple antibiotic-resistant strains and standard strains were collected from the hospital, and bacterial and fungal growth conditions were determined to establish a biofilm model. Different types and shapes of cleaning brushes were used to clean the biofilm, and the materials, shapes, and rotation speeds of the brush heads were compared. The biofilms were evaluated using microbiological isolation, culture, and scanning electron microscopy( SEM). Results: Regardless of the type and shape of the cleaning brush used, effective removal of biofilms was observed. However, subtle differences in material and shape of the brush head were shown to have a slight effect on the cleaning effectiveness. Specifically, brush heads with higher hardness and spherical shape performed better in removing biofilms, and high-speed rotation facilitated the removal of biofilms. Conclusion: Regardless of the type and shape of the cleaning brush, all were effective in removing biofilms. However, differences in materials and shapes of brush heads could slightly affect the cleaning effectiveness. Further studies and testing will be conducted to determine the most effective way to clean soft endoscopes.