激光捕获显微切割结合定量蛋白质组学技术筛选人肺鳞癌早期诊断标志物的中期报告.docxVIP

激光捕获显微切割结合定量蛋白质组学技术筛选人肺鳞癌早期诊断标志物的中期报告 中文摘要： 本研究旨在探讨激光捕获显微切割结合定量蛋白质组学技术的应用，筛选人肺鳞癌早期诊断标志物。采集15名早期肺鳞癌患者的组织标本和15名正常肺组织标本，通过激光捕获显微切割将组织蛋白质分离，然后用液相色谱-串联质谱（LC-MS/MS）进行定量蛋白质组学分析。通过分析蛋白质谱图，探讨肺鳞癌早期的特异性标志物，并验证其临床应用前景。 结果表明，与正常肺组织相比，肺鳞癌组织中有大量的特定蛋白质，如KRT5、KRT6A、KRT14等角化细胞特异性蛋白质，同时还有许多调节细胞增殖、诱导细胞凋亡的蛋白质，如FASN、CDK4、CDC42等。进一步分析表明，KRT5 是最具有诊断价值的标志物，其灵敏度和特异性均高达80%以上。 本研究初步筛选出了潜在的肺鳞癌早期诊断标志物，并明确了其临床应用前景。需要进一步拓展样本数量，并加强对蛋白质调控网络的研究，以提高诊断准确率，为临床治疗提供科学依据。 Abstract: The aim of this study was to explore the application of laser capture microdissection (LCM) combined with quantitative proteomics to screen early diagnostic biomarkers for lung squamous cell carcinoma (LSCC). Tissue samples were collected from 15 early-stage LSCC patients and 15 normal lung tissues. The tissue proteins were separated by LCM, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. By analyzing the proteome, specific biomarkers for early-stage LSCC were identified and their clinical application prospects were evaluated. The results showed that there were a large number of specific proteins in LSCC tissues compared with normal lung tissues, such as keratin 5 (KRT5), keratin 6A (KRT6A), and keratin 14 (KRT14), which are specific proteins of keratinocyte. There were also many proteins that regulate cell proliferation and induce cell apoptosis, such as fatty acid synthase (FASN), cyclin-dependent kinase 4 (CDK4), and cell division control protein 42 homolog (CDC42). Further analysis showed that KRT5 was the most valuable diagnostic biomarker, with sensitivity and specificity both above 80%. In this study, potential early diagnostic biomarkers for LSCC were preliminarily screened, and their clinical application prospects were clarified. Further expansion of sample size and strengthening the research on protein regulatory networks are needed to improve diagnostic accuracy and provide scientific basis for clinical treatment.