MALDI飞行时间质谱使用前准备.pdf

CHEM 5181 Laboratory #1 – MALDI Time-of-Flight Mass Spectrometry (Pre-lab) Introduction: In this lab you will analyze a mixture of three peptides using a matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometer (TOFMS) from PerSeptive Biosystems (now Applied Biosystems). You will investigate how different matrices affect the outcome of a MALDI spectrum. Then you will manipulate various parameters of the instrument to see what effect they have on the resulting spectra (e.g., ion time of flight, resolution, signal to noise). You wil