低温NMDA受体拮抗剂MK-801逆行灌注对猪脊髓损伤的保护作用的中期报告.docxVIP

低温NMDA受体拮抗剂MK-801逆行灌注对猪脊髓损伤的保护作用的中期报告 中文摘要： 目的：本研究探讨低温NMDA受体拮抗剂MK-801逆行灌注对猪脊髓损伤的保护作用及其机制。 方法：30只健康猪随机分为对照组和低温组，在T10上进行脊髓压迫造成损伤，实验动物于脊髓损伤后6小时进行MK-801逆行灌注处理，对比两组动物的神经功能、脊髓病理、脊髓血流动力学和脊髓中NO及SOD浓度的变化。 结果：与对照组相比，低温组的神经功能明显恢复、脊髓组织病理损伤缓解、脊髓血流动力学改善、脊髓中NO含量降低、SOD含量提高。 结论：低温NMDA受体拮抗剂MK-801逆行灌注具有对猪脊髓损伤的保护作用，可能与减少氧化应激反应有关。 英文摘要： Objective: To explore the protective effect and mechanism of low-temperature NMDA receptor antagonist MK-801 retrograde perfusion on spinal cord injury in pigs. Methods: Thirty healthy pigs were randomly divided into control group and low-temperature group. Spinal cord injury was performed at T10, and the experimental animals received MK-801 retrograde perfusion 6 hours after spinal cord injury. The changes of nerve function, spinal cord pathology, spinal cord hemodynamics, NO and SOD concentrations in spinal cord were compared between the two groups. Results: Compared with the control group, the low-temperature group had significant improvement in nerve function, relief of spinal cord tissue pathology, improvement of spinal cord hemodynamics, reduction of NO content in spinal cord, and increase of SOD content. Conclusion: Low-temperature NMDA receptor antagonist MK-801 retrograde perfusion has a protective effect on spinal cord injury in pigs, which may be related to the reduction of oxidative stress response.