醋酸纤维素膜电泳分离血浆蛋白(英文版).pptxVIP

Disciplines in the laboratory：Must wear white coat and don’t be late.Must keep quiet when you are in the laboratory.Don’t touch any experimental apparatus without my permission.An assistant needed. Collect the experimental reports and ask two students on duty to clean the lab after class every time. The instructor will check the laboratory at last. Five scores will be lost in your finial examination if you were late or did not clean the lab each time. Experimental report1. Experimental principle2. Experimental materials (optional)3. Experimental procedure4. Results and analysis or Results and CalculationIf you get a membrane (picture), please paste it on the lab notebook. Describe the membrane (picture) and try to explain the possible reason why you get the result;If you get the mathematic data, please do some calculation. 5. ConclusionUse one or two sentences to summarize your experiment. The experimental title Separation of plasma?proteinsby cellulose acetate membrane electrophoresis ElectrophoresisCharged particles move to the electrode of verse charge in the electric field.Particles: bio-macromolecule. e.g.DNA, RNA or protein. Direction: If particles carry negative charge, it will move toward positive electrode. If particles carry positive charge, it will move toward electrode.negative Cellulose acetate membrane A kind of material that has the net-like structure.The special structure can retard the proteins by the molecular weight. Protein larger, moves slower in the electric field. Proteins in the plasma?Thousands of proteins in the plasma.The main proteins are albumin, α1-globulin, α2-globulin, β-globulin, fibrinogen and γ-globulin. These proteins can be separated by cellulose acetate membrane electrophoresis. ?1. Experimental?principleThe?isoelectric point?(pI), is the?pH?at which a particular?molecule?carries no net?electrical charge?in the?environmental buffer. ?1. Experimental?principle Different proteins in plasma have different isoelectric?points