分子生物学学习资料:MB answers 14.docVIP

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AnswerstoWeaverendofchapterquestions

Chapter14MessengerRNAProcessingI:Splicing

1.DescribeandgivetheresultsofanR-loopingexperimentthatdemonstratesthatanintronistranscribed.

RefertoFig.14.3.InR-loopingexperiments,RNAishybridizedtoitsDNAtemplate.Whenthemouse?-globinmRNAprecursorwashybridizedtoaclonedmouse?-globingene(DNA),asmoothhybridwasformed(asopposedtowhenthematuremRNAwasused),indicatingthattheintronsarepresentintheoriginalRNAmade(thepre-mRNA).

2.Diagramthelariatmechanismofsplicing.

RefertoFig.14.

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