分子生物学学习资料:MB answers 04.docVIP

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AnswerstoWeaverendofchapterquestions

Chapter4MolecularCloningMethods

AluI bluntends.

BglII 4bp5’overhang

ClaI 2bp5’overhang

KpnI 4bp3’overhang

PvuI 2bp3’overhang

MboI 4bp5’overhang

NotI 4bp5’overhang

InordertoproducemanycopiesofaDNAfragmentwetypicallyreplicateitinanorganismsuchasE.coli.VectorssuchasplasmidshaveanoriginofreplicationandbyattachingaDNAfragmenttotheplasmidtheforeignDNAwillalsobereplicated.

CloningaDNAfragmentintothePstIsiteofpBR322requiresthattheDNAfragmentwewanttoclonehave

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