红莲型不育系与温敏型不育系杂交后代花粉育性的遗传学研究的开题报告.docxVIP

红莲型不育系与温敏型不育系杂交后代花粉育性的遗传学研究的开题报告

Title:GeneticStudyofPollenFertilityinHybridsofRed-andTemperature-SensitiveMaleSterileLines

Introduction:

Inhybridbreeding,malesterilelinesarewidelyusedfortheproductionofhybridseeds.Twocommontypesofmalesterilityarered-andtemperature-sensitivemalesterilityinplants.Thered-andtemperature-sensitivemalesterilelineshavebeenusedsuccessfullyinmanycrops,includingrice,wheat,andmaize.However,thegeneticbasisoftheirmalesterilityandpollenfertilityisstillunclear.Thisstudyaimstoinvestigatetheinheritanceofpollenfertilityinthehybridsofthesetwomalesterilelinesinmaize.

Objectives:

1.Toevaluatethepollenfertilityofthehybridsbetweenred-andtemperature-sensitivemalesterilelinesinmaize.

2.Toanalyzetheinheritanceofpollenfertilityinthehybridsusinggeneticmethods.

3.Toexplorethepotentialgeneticfactorsthataffectpollenfertilityinthehybrids.

Methods:

1.Crossingred-andtemperature-sensitivemalesterilelinestoproduceF1hybrids.

2.ObservingthephenotypeofpollengrainsunderamicroscopetodeterminepollenfertilityintheF1hybrids.

3.DeterminingtheinheritanceofpollenfertilityintheF1hybridsbyanalyzingthepollenfertilityofF2andbackcross(BC1)generations.

4.Usingmolecularmarkerstoidentifythegeneticfactorsassociatedwithpollenfertilityinthehybrids.

ExpectedResults:

1.Thehybridsbetweenred-andtemperature-sensitivemalesterilelineswillexhibitdifferentlevelsofpollenfertility.

2.Theinheritanceofpollenfertilityinthehybridswillbecontrolledbymultiplegeneswithadditiveeffects.

3.Someofthegeneticfactorsassociatedwithpollenfertilityinthehybridswillbeidentifiedandcharacterized.

Significance:

Understandingthegeneticsofpollenfertilityinthehybridsofred-andtemperature-sensitivemalesterilelineswillprovidevaluableinsightsintothemechanisms