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纳米金在生物监测上的应用
OpticalmethodsLSPRabsorptionFluorescenceemissionLightscattering(in/elastic)AuNPsenhanceshiftquenchenhance
LSPRabsorptionFig1.IllustrationofLSPRabsorptiondetectionofanalytesattachedonthesurfaceofAuNPs.Annu.Rev.Phy.Chem.2007,4,267-197.J.Chem.Phys.1980,73:3023-3037.
LSPRabsorptionFig2.TheplasmonresonanceofmetalNPisdependentonthesize,shapeandmaterial.
12FluorescenceemissionFig3.TheplasmonresonanceofmetalNPisdependentonthesize,shapeandmaterial.Fig4.SchematicrepresentationoftheCu2+sensorbasedonmodulationofthephotoluminescencequenchingofthechromophorebyAuNPs.Adv.Mater.2005,17,2811-2815.Fluorescencequenching纳米金淬灭荧光
FluorescenceemissionFluorescenceenhancementFig5.TheplasmonresonanceofmetalNPisdependentonthesize,shapeandmaterial.Fig6.Comparativeemissionspectraofafluorescentcore–shellAg@SiO2nanoparticleandacontrolnanoparticlecontainingnoAg.金属增强荧光(Metal-enhancedfluorescence,MEF)J.Phys.Chem.C,2009,113,12095-12100.
Lightscattering动态光散射(Dynamiclightscattering,DLS)Fig7.Bymonitoringthechangeinthescatterpattern,thediameterofthesolidnanoparticlescanbededuced.瑞利散射公式Stokes-Einstein公式
Fig8.Real-timemonitoringoftargetanalytebindingtotheAuNPsusingNanoDLayTM.Fig9.Calculationsofthecontributionsofscatteringandadsorptiontothetotalextinctioncross-sectionofAuNPsofdifferentsizesattheirplasmonbandposition.Talanta,2011,83,1580-1585.PLoSOne,2011,6(5),e19605(openaccess).
LightscatteringFig10.IllustrationofnormalRamanscatteringandSERS(Surface-enhancedRamanscattering).外表增强拉曼散射(SERS)Fig11.DifferentdetactionmethodsofplasmonicNPs(e.g.AuNPs)asSERSsubstrate.Small,2015,11,28,3395–3406.
SummaryAPros:mature,simple,wide,labelfree;Cons:relativelylowsensitivity,interferenceLSPRAbsorptionCPros:simple,sensitive,wide,stable;Cons
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