ASTM E3029-15美国材料与试验协会标准.pdfVIP

Designation:E3029−15

StandardPracticefor

DeterminingRelativeSpectralCorrectionFactorsfor

EmissionSignalofFluorescenceSpectrometers1

ThisstandardisissuedunderthefixeddesignationE3029;thenumberimmediatelyfollowingthedesignationindicatestheyearof

originaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.A

superscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.

1.ScopeE578TestMethodforLinearityofFluorescenceMeasuring

1.1Thispractice(1)2describesthreemethodsfordetermin-Systems

ingtherelativespectralcorrectionfactorsforgrating-basedE2719GuideforFluorescence—InstrumentCalibrationand

fluorescencespectrometersintheultraviolet-visiblespectralQualification

range.Thesemethodsareintendedforinstrumentswitha

0°/90°transmittingsamplegeometry.Eachmethodusesdif-3.SignificanceandUse(Intro)

ferenttypesoftransferstandards,including1)acalibratedlight

3.1Calibrationoftheresponsivityofthedetectionsystem

source(CS),2)acalibrateddetector(CD)andacalibrated

foremission(EM)asafunctionofEMwavelength(λEM),also

diffusereflector(CR),and3)certifiedreferencematerials

referredtoasspectralcorrectionofemission,isnecessaryfor

(CRMs).Thewavelengthregion