MCE稳定同位素标记物在代谢组学研究中的应用.docx

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(MFA)

13C 15N

[10]

Metabolite

extraction

BiofluidsCellsandmedia

Tissues

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Metabolomicdataacquistion

Glucose

Acetyl-CoA

Oxaloacetate

Citrate

Malate

3

TCAcycle

log

-

Fumarate

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Functionalandmechanisticvaildation

Metabolicfluxanalysis

VolcanoPlot

Heatmap

Metabolomicdataanalysis

Pyrimidinemetabolism

Pathwayimpact

Metabolicpathwayanalysis

Figure4.WorkflowofLC-MSbasedmetabolomics[10]

10

MCE

MasterofBioactiveMolecules

NADPH

(OxPPP)THF

(IDH1)

(MEI)

NADPH

NADPH

DNA

(OxPPP)

(Non-OxPPP)

M

(1-13Cglucose

12-13Cglucose)

(13C)

13CO2

OxPPP

1-13Cglucose

M+1

Non-OxPPP

5

12-13Cglucose

Non-OxPPP

M+1M+2

M+2

5[11][12]

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[1-C]glucosefeeding

D-Glucose

Oxidative

C1

PPP

G6P

G-1,5--6P

6PG

C1

C1

C1

G3P

F6P

Ru5P

Ribose

TK

C1

C1

Non-xidative

PPP

Histidine

Xu5P

R5P

TK

E4P

G3P

TA

63

%

C1

[1,2-C]glucosefeeding

C2

D-Glucose

Oxidative

C1

C1

C1

6PGC2

PPP

G6P

C2

G-1,5--6P

C1

C1

C1C2

C1

G3P

C2

Non-xidativeF6PC2

Ru5P

Ribose

PPP

TK

C1

C1

C2

C2

Xu5P

R5P

Histidine

E4P

G3P

TK

TA

63

Legends

OxPPP

None-OxPPP

1.0

[C]

[C]

Absenceofgene

ProportionalMIDs

0.8

0.6

0.4

0.2

0

m0

m1

m2

m3

m4

+LVP]

[C1-6]

Legends

OxPPP

None-OxPPP

[C]

1.0

[C]

Absenceofgene

0.8

0.6

0.4

0.2

0

m0

m1

m2

m3

m4

+LVP]

[C1-6]

Figure5.Movementof13ClabelthroughOxPPPandnon-OxPPPinR.solanacearumF1C1[11]

11

MCE

MasterofBioactiveMolecules

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References:

[1]

PlantSci.2016Aug;249:59-69.

[2]BiotechnologyAdvances.2018,1-14.

[3]

NatBiotechnol.2017Jun7;35(6):493-494.

@-0HGKHP?

[5]JAmSocMassSpectrom.2021Jun2;32(6):1538-1544.

[6]Anal.Chem.2010,82,3023–3031.

[7]

JChromatogrA.2020Dec20;1634:461715.

[8]Foods.2021Mar9;10(3):567.

[9]

Pharmazie.2019Nov1;74(11):688-693.

[10]PharmacolTher.2021Aug;224:107827.

[11]CurrOpinBiotechnol.2020Aug;6