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ANALYTICALBIOCHEMISTRY100,201-220(1979)
REVIEW
ReviewoftheFolinPhenolProteinQuantitationMethodofLowry,
Rosebrough,FarrandRandall
GARYL.PETERSON
DepartmentofPharmacology,UniversityofWisconsinMedicalSchool,Madison,Wisconsin53706
ReceivedJuly19,1979
Owingtoitssimplicity,sensitivityandcopingwiththeseinterferingsubstances,as
precision,themethodofLowry,Rose-wellastheotherdisadvantages(slowreac-
brough,Farr,andRandall(1)isthemosttionrates,reagentinstability,nonlinearity)
widelyusedprocedureforthequantitativearethenreviewed.Finally,themethodof
determinationofprotein.Lowryandco-Lowryetal.iscomparedwithothermethods
workerscombinedtheuseofcopper,asofproteinanalysisinthecontextofa
suggestedbyHerriott(2),withtheFolinstandardlaboratoryprocedure.
phenolmethod,whichoriginatedfromthe
workofWu(3),toproduceamorereliableREACTIONMECHANISM
andsensitiveproteinassay.Theprincipal
disadvantageofthismethodisitslackofThemethodofLowryetal.isbasedon
specificity.ManysubstancesarenowknowntheFolinphenolreagentofFolinand
tointerferew
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