两种植物精油对扩展青霉菌体生长和产毒量的影响的开题报告.docxVIP

两种植物精油对扩展青霉菌体生长和产毒量的影响的开题报告

Title:TheeffectsoftwoplantessentialoilsonthegrowthextensionandtoxinproductionofAspergillusflavus.

Introduction:

Aspergillusflavusisacommonfungusthatcancontaminatefoodproductssuchasgrains,nuts,andcorn.Itproducestoxiccompoundscalledaflatoxins,whichareknowntohaveharmfuleffectsonhumanhealthandcancauselivercancer.Essentialoilsderivedfromplantshavebeenfoundtoexhibitantimicrobialandantifungalproperties,andcouldpotentiallybeusedtoinhibitthegrowthandaflatoxinproductionofA.flavus.Inthisstudy,weaimtoinvestigatetheeffectsoftwoplantessentialoilsonthegrowthextensionandtoxinproductionofA.flavus.

Objectives:

1.ToevaluatetheinhibitoryeffectsoftwoplantessentialoilsonthegrowthextensionofA.flavus.

2.ToassesstheimpactoftheplantessentialoilsontheproductionofaflatoxinsbyA.flavus.

Methods:

1.Preparationofplantessentialoils:Twoessentialoils,namelyteatreeoilandoreganooil,willbeusedinthisstudy.Theoilswillbeextractedfromtherespectiveplantmaterialsusingsteamdistillation.

2.Assayforfungalgrowthextension:A.flavuswillbeculturedinPotatoDextroseAgar(PDA)mediumandtreatedwithdifferentconcentrationsofthetwoplantessentialoils.Theextensionoffungalgrowthwillbemonitoreddaily.

3.Assayforaflatoxinproduction:A.flavuswillbeculturedinYeastExtractSucrose(YES)mediumthathasbeensupplementedwiththeplantessentialoils.AflatoxinproductionwillbemeasuredusingHigh-PerformanceLiquidChromatography(HPLC).

Expectedresults:

WeexpectthattheplantessentialoilswillexhibitinhibitoryeffectsonthegrowthextensionofA.flavus.Furthermore,wehypothesizethattheseoilswillalsosignificantlyreducetheproductionofaflatoxinsbyA.flavus.

Conclusion:

Thisstudywillprovidevaluableinsightsintothepotentialofusingplantessentialoilsasnatura