对小鼠黑色素瘤细胞肺转移的抑制作用mGM.pdfVIP

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对小鼠黑色素瘤细胞肺转移的抑制作用mGM

作者：田蓉，程绍辉，于继云，李巍，刘玉峰

【关键词】黑色素瘤

InhibitionoflungmetastasisofB16melanomacells

transfectedwithmurinegranulocytemacrophage

colonystimulatingfactorinmice

【Abstract】AIM:Toinvestigatetheinhibitionoflung

metastasisofmelanomabyatumorvaccineofB16melanomacells

transfectedwithmurinegranulocytemacrophage

colonystimulatingfactor(mGMCSF).METHODS:Themurinemelanoma

celllineB16wastransfectedwiththerecombinantplasmid

containingmGMCSF.AcelllinestablyexpressingmGMCSFwas

obtainedbyG418selection.TheBalb/cmicewereadministered

i.v.withB16cellstransfectedwithorwithoutmGMCSF

respectivelyandthelungmetastasiswasexamined2weekslater.

RESULTS:TherecombinantcelllineB16TRwasestablishedafter

mGMCSFtranfectionandtheexpressionofmGMCSFwasconfirmed

byRTPCR.Thenumberoflungmetastaticlesionsofmicetreated

withB16TRwassignificantlyfewerthanthatofmicetreatedwith

B16(n=5,125.8±34.3vs27.8±24.0,Plt;0.01).CONCLUSION:

ThemGMCSFtransfectioncansignificantlysuppressthelung

metastasisofmelanomainmice.TheGMCSFcanbeapromising

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adjuvantintheimmunotherapyofmelanoma.

【Keywords】melanoma;mice;granulocytemacrophage

colonystimulatingfactor;lungneoplasms/secondary;cancer

vaccines

【摘要】目的：探讨小鼠粒细胞巨噬细胞－集落刺激因子

（mGMCSF）基因转导对B16黑色素瘤细胞肺转移的抑制效果.方法：

应用脂质体将含有mGMCSF基因的真核表达载体转染B16小鼠黑色素瘤

细胞，经G418加压筛选后，挑选表达mGMCSF基因的B16黑色素瘤细

胞.观察转导或未转导mGMCSF基因的B16黑色素瘤细胞在Balb/c小

鼠体内的肺转移.结果：转导mGM