肾透明细胞癌相关基因及通路的筛选及生物信息学分析 .pdfVIP

肾透明细胞癌相关基因及通路的筛选及生物信息学分析

王永志;王锦星;王行环

【摘要】ObjectiveToidentifythepotentialtargetgenesandsignal

pathwaysinthedevelopmentofrenalclearcellcarcinoma(ccRCC)by

bioinformaticsanalysis.MethodsThedataofGSE53000weredownloaded

fromtheGEOwebsite.Thedifferentialexpressiongenes(DEGs)between

ccRCCandnormalrenaltissuewereidentifiedwithRSoftware.The

clusteringanalysisandfunctionalenrichmentanalysisoftheseDEGswere

thenperformed,followedbyprotein-proteininteraction(PPI)networkand

pathwayrelationnetwork.Meanwhile,themostsignificantmodulebased

onPPInetworkwasselected,andthegenesinthemodulewereidentified

ashubgenes.ResultsComparedwithnormalrenaltissue,ccRCChadatotal

of1175geneswhichweredifferentiallyexpressed.Amongthem,533were

up-regulatedand642weredown-regulated.Genomicanalysisshowedthat

the10biologicalprocesseswiththehighestcorrelationwiththese

differentialgeneswerecelladhesion,leukocytemigrationand

inflammatoryresponse.KEGGanalysisshowedthatthe10mostsignificant

signalpathwaysincludedcomplementandcoagulationcascade,adhesion

moleculepathwayandcytokine-cytokinereceptorinteraction

pathway.Altogether20throttlegeneswereselectedfromthePPI

network,12ofwhichwereassociatedwiththeinflammatoryresponse

process.ConclusionTheidentified12genesarelikelytoplayanimportant

roleinthepathogenesisofccRCCandmaybeusedasatherapeutictarget

and/ordiagnosticmarkerforccRCCinthefuture.%目的运用生物信息学方

法鉴别肾透明细胞癌(ccRCC)发生发展过程中的潜在靶基因及信号通路.方法从

GEO网站下载基因芯片GSE53000的数据集,用R软件识别肾透明细胞癌和正常

肾组织之间的差异表达基因,并对差异表达基因进行层次聚类分析.然后用DAVID

网站对差异基因进行基因本体(Geneontology)分析和KEGG通路富集分析,最后

用STRING网站和Cytoscape软件构建蛋白-蛋白互作(PPI)网络,同时从中筛选出

最具有意义的模块,并将其中的基因作为枢纽基因.结果与正常肾组织相比,共计1

175个基因在肾透明细胞癌中差异表达,其中533个基因高表达,642个基因低表达.

基因本体分析显示与这些差异基因相关度最高的10个生物学过程分别是细胞粘附、

白细胞迁移以及炎症反应等,KEGG分析结果显示最显著的10条信号通路包括补体

和凝血级联